Abstract:Objective To observe the pathology changes in lungs lesions of captive rhesus monkey，explore the distribution rule and pathological features of diseases in respiratory system． Methods We postmortem 155 nature dead monkeys from 2 ～ 20 years which were bred in institutes in Yunnan provinces． Monkeys were divided into young，adult and aged groups according to their age． The pathology changes of lung were investigated by routine histopathological technique and the analysis of chi-square test was performed． Results Of 155 monkeys，59 samples have lung lesions，the ratio was 38. 06% ． the main lesion include pulmonary edema，intralveolar hemorrhage，lobar pneumonia，alveolar organization，lobular pneumonia， chronic bronchitis， bronchiectasis， interstitial pneumonia， granulomatosis of lung， emphysema，pulmonary abscess，pleurisy． The kinds and the frequency of lesions are different in different age groups． Conclusions The incidence rates of diseases of lung of breeding rhesus macaques is higher and its interference effect should be seriously taken． Some pathological changes of lung are different in diverse age groups． The results of our test enrich the data of the captive monkeys，and it is useful in quality control of captive monkeys and animal tests．

Abstract:Objective To obtain the basic data of biological parameters of F1 of a self-established C57-ras transgenic mice． Methods The blood samples were collected and tested for physiological values using MEK 27222K hematology analyzer and biochemical values using Olympus AU400 auto 2 analyzer． The weight of organs and bodies were determined with electronic balance and the data were analyzed statistically． Results The MCHC of F1 female transgenic mice and non-transgenic mice had extremely significant difference ( P ＜ 0. 01 ) . The PLT，PCT，EOS% ，NEUT， NEUT% ，LYM% of CB6F1-Tg male mice and non-transgenic mice had significant difference ( P ＜ 0. 05 ) ，and that of NEUT% ，LYM% had extremely significant difference (P ＜ 0. 01) ． Among the sera biochemical data，ALT，TP，ALB had significant difference ( P ＜ 0. 05 ) ． The statistical analyzing results of organ weight and coefficient show that had no extremely significant difference (P ＞ 0. 05) ． TG had extremely significant difference (P ＜ 0. 01) ． Conclusions The F1 of new-established mouse model has been obtained the biological characters with the normal C57BL /6 mice; this may be favorable for its usage in the pre-clinical safety evaluation．

Abstract:Objective To investigate the distribution of Progesterone Receptor ( PR) in murine ovary，fallopian tube and uterus between the mice treated by estrus synchronization of PMSG ( pregnant mare serum gonadotropin) and of FSH ( follicle stimulating hormone) ． Methods Total 10 KM female mice were divided into two groups: PMSG and FSH treatment group，after the injection of these hormones for 48 h，all of their ovaries，oviducts and uterus were fixed by paraform，and stained by immunohistochemical staining to observe and analyze the distribution of PR in these tissues．Results The positively stained cell number in the primary and secondary ovarian follicles of the PMSG treatment group，were significantly higher than those of the FSH one(P ＜ 0. 05) ，the average absorbance in the ovarian follicles of the PMSG treatment group was significantly higher than that of the FSH one ( P ＜ 0. 05 ) ． In addition，The positively stained cell number in oviduct of the PMSG treatment group was significantly higher than those of the FSH group(P ＜ 0. 05) ，but the average absorbance is reversed． In endometrial epithelium and glandular epithelium，the positively stained cell number of the PMSG treatment group was significantly higher than that in the FSH one ( P ＜ 0. 05 ) ，the average absorbance in endometrial stroma and endometrium of the PMSG treatment group was significantly lower than that of the FSH one( P ＜0. 05) ． Conclusions PMSG and FSH may induce varying degrees of impact on the distribution of PR in murine ovaries，oviducts and uterus，the expression of PR of the PMSG treatment group was significantly higher than that of the FSH one．

Abstract:Objective To compared the ratios of fluorescence labeling cells to the lin( － ) c-kit( + ) Sca-1 + ( LSK)stem cells from the five transgenic mouse lines，selected the mouse lines of totally fluorescence labeled，for tracking the hematopoietic stem cells and in vivo fluorescence imaging analysis． Methods The expressions of the fluorescent protein in vivo were observed under in vivo fluorescence imaging system，the expression of those genes in the stem cells were investigated with flow cytometry． Results The fluorescent protein was expressed in most tissues in the five transgenic mice． The fluorescent protein expressions were observed in almost all LSK stem cells of C57BL /6J-TgN( CAG-DsRed-1) ZLFILAS and C57BL /6J-TgN ( CAG-EGFP-1 ) ZLFILAS． Conclusions Two transgenic mouse lines were indicated in which LSK stem cells were marked completely by red or green fluorescence protein separately，suggesting the two mouse lines may be useful tools for in vivo tracking the hematopoietic stem cells．

Abstract:Objective To study the effect of probucol on the oxidative stress of kidney in diabetic rats． Methods Diabetes mellitus rat model was induced by streptozotocin( STZ) ． 30 Wistar rats were randomly divided into three groups:normal control group ( NC) ，diabetes mellitus group ( DM) ，probucol-treated diabetes mellitus group ( DP ) ． After 8 weeks，body weight and kidney weight ( calculate kidney weight / body weight) ，urine albumin excretion rate(UAER) and biochemical indicators including blood glucose(BG) ，total cholesterol ( TC) triglycerides( TG) ，serum creatinine( SCr) ，blood urea nitrogen(BUN) were measured; The levels of maleic dialdehyde (MDA) as well as the activities of antioxidant enzymes including superoxide dismutase ( SOD) ，catalase ( CAT) ，glutathione peroxidase ( GSH-Px) in the renal tissue were determined; the renal tissue was observed by light microscopy: renal morphology parameter including glomerular area and glomerular volume by PAS staining． Results Kidney weight，ratio of kidney weight to body weight，UAER，TC，TG，SCr，BUN and glomerular area，glomerular volume on histological examination of the kidney were significantly increased in DM group rats than those in NC group． The changes described above were attenuated by treatment with probucol ( P ＜ 0．05) ． Elevated MDA level as well as decreased activities of SOD，CAT and GSH-Px in diabetic renal tissue were also remitted by probucol ( P ＜ 0. 05 ) ． Conclusions The renoprotective mechanism of probucol may be at least partly correlated with relieving oxidative stress in the renal tissue of diabetic rats．

Abstract:Objective To study the genetic quality for six strains of inbred mice in Liaoning Province by microsatellites technology． Methods Ten microsatellites DNA locis and primers were selected in Mouse Genome Database and pertinent literature，and its polymorphism information content was rich，to analyzed on genetic polymorphism of mouse by PCR Amplification and PAGE electrophoresis． Results The same site results of PCR amplification showed polymorphism in different strains， and showed monomorphism in same strains， ten microsatellites DNA locis were homozygous state for all mouse． The genetic distance among C57BL /10 and C57BL /6J was 0． 1021，and it was 0. 1635 and 0. 1614 between BALB / c with C57BL /10 and C57BL /6J． Conclusions It was a feasible way to analyzed genetic monitoring among inbred mouse strains by ten microsatellites DNA locis．

Abstract:Objective To determine and compare the blood parameters and blood biochemical parameters in F1 and F2 germ-free rats． Methods Germ-free rats were obtained by uterectomy，and the offspring were fed with artificial milk by hand in the germfree isolator． The blood parameters and blood biochemical parameters were detected by automatic hematology analyzers． Results The germ-free rats showed significant differences in the values of RBC，HGB，HCT，MCV，MCHC，RDW，MPV，NE% ，LYM% ，EO% ，BA% ，NE#，LYM#，EO#，BA#( P ＜ 0． 05) and ALT，ALB，LDH，CHO，TBIL，HDL-C，LDL-C，GLO，GLU，ALP，A /G，UA，P(P ＜ 0. 05) ． There are different blood values in different gender and age．Conclusion The gender and age may affect the blood parameters and blood biochemical parameters．

Abstract:Objective To explore the role of mir-153 in Alzheimer’s disease． Methods Detected the expression of mir-153 in the brain tissue of 3 months old APPswe /PSΔE9 mouse by means of microRNA array and Real-time PCR．Developed the stable transfection cell line that expressed mir-153 on a high level． Explored the expression of RTN4 protein by western-blot． Constructed the wild type and mutation type RTN4 3’UTR luciferase reporter vecter，cotransfected them with mir-153 expression vecter or negative control into 293T cell，detected the relative activity of Renilla Luciferase to check the binding site of mir-153 on RTN4’s mRNA． Results The result of microRNA arry and Real-time PCR all indicated in 3 months old APP swe /PSΔE9 mouse，the expression of mir-153 significantly decreased when compared to the wild contro on the same age． In stable transfection cell line expressing high level mir-153，the expression of RTN4 protein was downregulated． When compared to negative control / wild type RTN4 3’UTR contransfection group，Cotransfection mir-153 together with wild type RTN4 3’UTR． can strikingly shutdown the relative activity of Renilla Luciferase ( P ＜0. 05) ，however mir-153 / mutation type RTN4 3’UTR cotransfection group couldn’t reach any significant difference．Conclusions In 3 months old APPswe /PSΔE9 mouse’s brain，the expression of mir-153 is abnormal． Mir-153 can regulate the expression of RTN4 on protein level． The regulation effect of mir-153 on RTN4 is achieved by binding the specific site lie in 839-845 bp of RTN4 3’UTR．

Abstract:Objective To establish stable and reliable rat renal transplantation models of chronic rejection．Methods Wistar and SD rats were used as donors and recipients，left renal was removed from the donors． End-to-end anastomosis of inferior vena cava and renal vein，abdominal aorta and renal artery were operated． At 3，6，9 weeks，renal allografts were removed and postoperative complications and renection were observed． Results At 9 weeks，visible chronic rejection could be seen and some allograts suffered from hydronephrosis，while which had little relationship with rejection．Conclusions This method is feasible and reliable for chronic rejection of rat and it is an ideal model．

Abstract:Objective Chlamydia trachomatis infection is the most common sexually transmitted disease. The aim of this research is to set a standard system for determination of chlamydia load in tissues from infected animals． Methods Chlamydia trachomatis ( serovar E) was amplified in vitro for infection． The gene fragment of the chlamydia-specific gene OMP1 was cloned as standard． The quantity of chlamydia was determined based on the copy number of chlamydia genome by real time PCR． Results The results of real time PCR were linear when the copy number of OMP1 gene was within the range from 200 to 2 × 108． Unspecific amplification was not detected after adding mouse genome into the templates，nor was the amplification efficiency affected． Conclusion Real time PCR targeting chlamydia-specific gene OMP1 could be applied as a standard method to quantitatively analysis chlamydia in infected animal sample with adequate sensitivity and specificity．

Abstract:Objective To investigate the staining rule of several special staining methods on bone and joint staining and their value in morphological research of osteoarthritis． Methods 20 6-month old healthy New Zealand rabbits were randomly divided into normal group and osteoarthritis model group，10 in each group． The model rabbits were replicated into knee osteoarthritis model by modified Hulth method． The materials were drawn out 6 weeks after operation．The samples were fixed，decalcified，paraffin embedded and made into paraffin sections，then HE staining，Safranin-Fast Green staining，AB-PAS staining，toluidine blue staining，Van Gieson staining and Mallory staining were applied to observe the morphology of the tissue，and all of the staining methods were compared． Results HE staining showed normal tissue morphology of joints，pathological changes of osteoarthritis can be seen in articular cartilage and subchondral bone of model group; Safranin-Fast Green staining showed clear boundary of cartilage and subchondral bone ( cement line) ，and a clear tide line，the glycosaminoglycan in Cartilage matrix decreased and the fiber Increased; AB-PAS staining showed that glycosaminoglycan，especially the acid glycosaminoglycan reduced in osteoarthritis; toluidine blue staining showed the acid glycosaminoglycan reduced in the tissues of osteoarthritis; Van Gieson staining and Mallory staining showed collagen fibers of bone and joint tissues，but the boundaries of various tissues were not clear． Conclusion Application of HE staining，in combination with safranin-fast green staining and AB-PAS staining in osteoarthritis study may acquire more objective and comprehensive information on joint morphology．

Abstract:Objective To Modify the permanent bilateral common carotid artery ( CCA) occlusion ( 2VO) for vascular dementia (VD) rats model，in order to increase the survival rate of model animals． Methods Vascular dementia was established by the modified 2VO method ( ligate unilateral CCA at first，ligate another one 7 days later) and the traditional 2VO method，respectively，and compared the differences of the two kind of methods in survival rates of rats， learning and memory ability and pathological morphology． Results One week after the operations，the survival rate of modified model group animal (86. 7% ) was obviously higher than traditional model group (40. 0% ，P ＜ 0. 05) ． Morris water maze test results showed that memory abilities in both modified and traditional model group were decreased obviously compared with the sham operation group ( P ＜ 0. 05 ) ，and there was no significant difference between modified model group and traditional model group． The neurons in hippocampus in modified and traditional model rats were all shown significant degeneration，necrosis and apoptosis． Conclusion The modified 2VO method is an ideal way to establish rat model of VD．

Abstract:Objective To study the effect of ultrasonic cleaner( UC) on electrocardiogram( ECG) in anesthetized rats． Method The electrocardiogram was observed by RM6240 Multi-channel physiological signal acquisition decency via standard limb lead II in anesthetized rats when the ultrasonic cleaner was working for different time． Result When ultrasonic cleaner worked for 2 min，15min and 30min，heart rate( HR) of rats increased significantly and QRS complex interval in electrocardiogram was significantly shortened at the same time． But heart rate and QRS complex interval recovered after the UC stopped． Conclusion Ultrasonic cleaner at work produced recoverable adverse effect on electrocardiogram in rats． Therefore，ultrasonic cleaner should be placed as far as possible away from the rat breeding room．

Abstract:ObjectiveTo establish multiplex PCR assay for detects Brucellosis in laboratory canine and related biological products． Method These primers of multiplex PCR were designed according to the Omp2 gene of Brucellosis，and the PCR reaction was optimum，and then some samples were digested by three restriction enzyme for differentiate Brucellas． We also cloned PCR products and measured sequence after multiplex PCR to determine the accuracy of the assay． Then the specificity and sensitivity of the assay was tested． Results The target bands were successfully amplified，and these samples were successfully distinguished by digestion of the PCR product; It was 99% homology between the target segment by multiplex PCR and Brucellosis DNA sequence from GeneBank． The specificity of this method is better，1. 8 × 10 － 7 μg /ml samples can be detect． Conclusion The Brucella PCR detection and typing of multiple identification methods was established successful，the assay have good specificity and high sensitivity． The assay is important to ensure the quality of laboratory canine and protect the health of the animal keepers and laboratory personnels．

Abstract:Stem cell transplantation will be or have become an important method of treatmenting various diseases．The success rate of stem cell transplantation is closely related to the stem cell proliferation and amplified，the process of stem cell transplantation is regulating by many cytokines． The current studies found that the cytokines of interleukin family IL1，IL2，IL3，IL4，IL6，IL7，IL8，IL9，IL10，IL11，IL12 play a very important role in the process of regulating the stem cell mobilization． This review summary the biological activities and the their effect on the stem cell mobilization of thi part of the interleukin family menbers．

Abstract:Human immunodeficiency virus type 1 ( HIV-1) clade C is the most prevalent clade in the world，and simian-human immunodeficiency virus ( SHIV) encoding HIV-1 clade C env and by which the nonhuman primates model is made are efficient tools for investigation of human acquired immune deficiency syndrome ( AIDS ) ． SHIV-1157i and variations from SHIV-1157i can successfully infect rhesus macaques and pig-tailed macaques via mucosa and causing symptoms like AIDS eventually，and the slow process of onset in the in vivo model of rhesus macaques resulted from these SHIVs is similar to HIV-1 infection from human being． Therefore，to elucidate the pathogenesis of infection of rhesus macaques by SHIV-1157i and its variations is of great significance for investigating HIV infection and pathogenesis in human being，and for development of candidate AIDS vaccines targeting HIV-1 clade C env．

Abstract:Objective To investigate the epizoic parasites infection in tree shrew( tupaia belangeri chinensis) ，and to provide a basis for seting up the quality standard of tree shrew． Methods Sixty wild tree shrews were executed by dislocation，and took hair to production from oxter、ear、groin、round anus of wild tree shrews and F1 generation tree shrews， then detected the epizoic parasites under platoscope and microscope． Results The natural infectious rate of the epizoic parasites in wild tree shrew and F1 generation tree shrews were respectively louse ( 25% /6% )、flea ( 5% /0 )、cicadas (1. 6% /0)、bloodsucker(1. 6% /0)、infection with louse and flea(1. 6% /0) ． Conclusions The most of epizoic parasites identified in tree shrews were louse and fleas，secondly were cicadas and bloodsucker． As a whole，infectious rate of the wild tree shrews was obvious more than F1 generation tree shrews，and we gained good effect by taking a preventing and treating measures．

Abstract:Objective To investigate the potential infections of ten kinds of swine pathogens，including HCV，PRV，PRRSV，SIV，PPV，JEV，PCV2，Toxoplasma，chlamydial and Brucella in Tibet Minipigs． Methods Enzymelinked immunosorbent assay and serum agglutination test were used to detect antibodies of ten kinds of the swine diseases in blood samples，including ten samples from Tibet Minipigs sows of F0 generation which have been used swine fever vaccine， forty samples from 20-day-old suckling Tibet Minipigs and fifty samples from 3 ～ 5 months old Tibet Minipigs of F1 generation． Results The positive rate of HCV-Ab，PPV-Ab and JEV-Ab were 80. 0% 、80. 0% 、70. 0% in Tibet Minipigs sows，respectively; while 45. 0% 、25. 0% 、45. 0% in 20-day-old suckling Tibet Minipigs，respectively ;4. 0% 、76. 0% 、80. 0% in 3 ～ 5 months old Tibet Minipigs，respectively． The positive rate of PCV2-Ab is 60. 0% in Tibet Minipigs sows，5. 0% in 20-day-old suckling Tibet Minipigs，while 0% in 3 to 5 months old Tibet Minipigs． However，none of PRRSVAb，PRV-Ab，SIV-Ab，Toxo-Ab，chlamydial-Ab Brucella-Ab were detected in all Tibet Minipigs ． Conclusion In this study，PRRSV，PRV，SIV，Toxo，chlamydial and Brucella haven’t been found in Tibet Minipigs of different ages．

Abstract:Veterinary care is an important requirement for laboratory animal performance，especially for those animals with infectious pathogens，like monkeys infected with SIVs． They need clinical observations，co-infection control，management of treatment，welfare records and so on． Due to the particularity of housing and management of monkeys，theveterinary care may be varying acoording to the requirements of particular technical procedures in different researchs．