Abstract:Objective To prepare the rabbit polyclonal antibodies against seventeen mammals and to label the secondary antibodies with horseradish peroxidase for developing the serological test system of mammals． Methods IgGs of mammals were purified by precipitation with ammonium sulfate combining affinity chromatography using Protein A or Protein G． Rabbit anti-sera were prepared by repeatedly immunized rabbits with purified IgGs． The antibodies against mammal IgG in rabbit sera were detected by agarose double immunodiffusion and purified using Protein A affinity chromatography． The products were labeled using HRP by periodate oxidation method． And the activities of the labeled rabbit polyclonal antibodies against seventeen mammals were detected by ELISA． Western blots were performed to investigate the specificity of the seventeen rabbit anti-mammal IgGs-HRP． Results The IgGs from the seventeen mammal species were purified，including rhesus，tiger，brandt's vole，striped hamster，goral，guanaco，civet cat，crab-eating macaque，sika deer，mongolian gerbil，red deer，camel，hog deer，ibex，hamster，assam macaque，ferret． The titers of seventeen rabbit antisera were up to 1 ∶ 32 examined by agarose double immunodiffusion． Seventeen kinds of purified rabbit anti-mammalsecondary antibodies were labeled with HRP and their activities were up to 1 ∶ 2000 － 60000 examined by ELISA． Thespecific activities of the seventeen rabbit anti-mammal IgGs-HRP were indicated with western blot． Conclusion Rabbit polyclonal antibodies against seventeen mammals labeled with HRP were prepared successfully，it is an useful tool for developing the serological test system of mammals．

Abstract:Objective To complete sequence of mitochondrial ATPase8，ATPase6，COX3 of Mongolian Gerbil determined basing on the PCR products． The sequence was analysied in phylogenetic tree，nucleotide composition and genetic distance． Methods The primer was designed according to the published sequences． The PCR product were sequenced and determined． Combined with known ATPase8，ATPase6，COX3 sequence of other rodents nucleotide composition and genetic distance were analysed; phylogenetic tree was constructed by minimum-evolution ( ME) methods and UPGMA methods． Result The ATPase8，ATPase6，COX3 gene were cloned in Mongolian Gerbil，which have high homology with rat，mouse and hamster ( 76 ～ 98% ) ; it is higher genetic relationship with rat，mouse and hamster by phylogenetic analysis; the G content ( 6. 9% ，10. 7% ，15. 2% ) are similar with feature of mtDNA; the A + T content ( 68. 2% ，64. 1% ，59. 2% ) are similar with feature of mammalian genome． Conclusion The ATPase8，ATPase6，COX3， gene sequence of mongolian Gerbil was determined for the first time． We found that Mongolian Gerbil have close genetic relationship with rat，mouse and hamster in this research． This research is an important work for study on evolution，structure and function of the mongolian gerbil mitochondrion．

Abstract:Objective To establishment of human pancreatic cancer mouse model labeled with luciferase and evaluate bioluminescent imaging and ultrasound imaging for tumor burden and tumor progression in a mouse model of pancreatic cancer． Method The human pancreatic cancer cell line Capan-2，engineered for stable，high-level expression of luciferin ( LUC) ，was implanted into pancreas and subcutaneous tissue of nude mice． The tumors were allowed to grow over a period of one to several weeks during which time the mice were imaged using both bioluminescent imaging and ultrasound imaging to monitor tumor growth． Result The successful rate of orthotopic and subcutaneous transplantation was 75% and 100% separately． The whole-body optical imaging found that the fluorescence could be detected after 7 days of orthotopic transplantation． The size of tumor can be measured after 7 days of subcutaneous transplantation by ultrasound imaging but it can't be done in orthotopic transplantation． Otherwise the growth rate of tumor besides subcutaneous is about 3 times faster than pancreas． Conclusion Bioluminescent imaging is more suitable for monitoring the tumor at the early stage，and provides an ideal tool to further study the development of tumor and the mechanism of metastasis．

Abstract:Cricetulus barabensis; Albino mutant ( A: CHA) ; Physiological and biochemical of blood

Abstract:Objective To establish an animal of diet induced non-alcoholic fatty liver disease ( NAFLD) and hyperglycemia，and to investigate its features． Methods 64 rats were randomly divided into 2 groups． The control group ( fed with normal diet) 32，high glucose and high fat group ( fed with high sugar and high fat diet) 32，continuous feeding of 12 months． on the 3month、6months、9 months、12 month of the experiments，we observe animal weight，visceral fat weight; Comparing relevant in the blood lipid levels，blood sugar，inflammatory mediators aspects of biochemical indicators and observed histopathologically． Results Compared with the normal control group，weight and visceral fat weightof the animals of high glucose and high fat groups increased obviously; the level of ALT，FFA，LPS，TNF，FPG，FINS and HOMAIR of hig sucrose and high fat groups of every phases were higer，but the level of HOMA-beta of 6H group rats first showed the compensatory increase，then progressive decrease in sequence． The pathological and histological display liver produces serious steatosis，fatty liver and hepatitis，fibrosis and cirrhosis occurs; Over time progress islet gradually atrophic， accompanied by inflammatory infiltrate; Fat cells gradually increasing and accompanied by inflammatory infiltrate． Conclusion High sugar and fat diet can establish rats models of NAFLD and hyperglycemia，this model can be play important role in NAFLD and related diabetes research．

Abstract:Objective To clone the prokaryotic expression of leptin mature peptide and a partial leptin receptor extracellular domain in Tibet minipig． Methods Two pairs of primers，amplifying the 64 － 504 bp leptin mature peptide coding region，and 1654 － 2319 bp leptin receptor ( OBR) extracellular domain ( ECD) ，were designed and synthesized according to the sequences of swine leptin ( GenBank No． GQ240885. 1) and OBR ( GenBank No． AF167719. 1) genes． Total RNA was extracted from the Tibet minipig liver tissue，and was used as the template in RT-PCR to amplify the cDNA fragments coding leptin mature peptide and OBR ECD． The two fragments were then cloned into the pMD18 -T vector and the resultant plasmid was transformed into competent E． coli strain DH5α． The fragments were digested and cloned into the BamHⅠand HindⅢ sites of the expression vector pRSETA to form the recombinant plasmids pR-OB and pR-OBR-a，which were subsequently transformed into E． coli strain BL21 ( DE3 ) for expression of the recombinant proteins． Results The transformed bacteria pR-OB and pR-OBR-a were induced with IPTG and produced recombinant proteins of the relative molecular mass of 17. 5 × 103 and 27 × 103，respectively． Conclusions pR-OB and pR-OBR-a can express recombinant porcine leptin mature peptide and a partial OBR ECD of Tibet minipig in E． coli． strain BL21( DE3) ． This work provided the basis for further research on pig leptin and OBR．

Abstract:Macaca mulatta; Shed; Nutriology; Trace element; Amino acid

Abstract:Objective To investigate haemostasis effect of thrombase retroperfusion through ureteral catheter on nephrorrhagia rats． Methods After renal biopsy with systemic heparinization，the models of nephrorrhagia rats with gross hematuria had been established，32 SD rats weighing 350 ～ 400g were divided into 4 groups randomly ( 8 rats in each group) ． All rats were retroperfused by three different doses of thrombase or NS through ureteral catheter respectively． Lab index: Urinary red cell，blood pigment，serum creatinine，bleeding time( BT) and clotting time( CT) were detected in all rats before retroperfusion，5 min and 40 min after retroperfusion． Results ①Compared with NS group，the level of red blood cell count in urine of the other 3 groups was significantly different respectively( P ＜ 0. 05) ，and the level of abovementioned index among high-dose group and middle-dose group was lower than low-dose group ( P ＜ 0. 05 ) ． While the difference of blood pigment between different distinctive dose groups and NS group was significant in 40 min after retroperfusion( P ＜ 0. 05) ． The level of above-mentioned index among high-dose group ( 500U /mL) and middling-dose group ( 250 U /mL) was distinguished higher versus low-dose group respectively ． ② Among 4 groups，the difference of bleeding time( BT) 、clotting time( CT) and serum creatinine between the former and the latter was not significant( P ＞ 0． O5 ) ．Conclusion All three different doses of thrombase had haemostasis effect on renal hemorrhage rats by retroperfusion through ureteral catheter，and the effects were related to the medicament and the dosage; while retroperfusion thrombase did not affect BT，CT and the level of serum creatinine of rats．

Abstract:Objective Analyzed the presence of transcription variant of Estrogen receptor of Beagle dogs．Methods According to the reported complete nucleotide sequence of estrogen receptor in NCBI，two pairs of primers were designed and synthesized． The Estrogen receptor cDNA was amplified from the total RNA of the Ovary， uterus，hypothalamus and pituitary of Beagle by reverse transcriptase PCR ( RT-PCR ) ． Then major bands were cloned and sequenced． Results Obtained full-length cDNA sequence of estrogen receptor of Beagle dogs，the major bands were cloned and sequenced ，the results show that the sequence is an Estrogen receptor of Beagle dogs． Conclusion The existence of the transcription variant of Estrogen receptor of Beagle dogs，which was different from ones of Beagle dogs that had been predicted in the online database，It needs further studying．

Abstract:Cheek pouch bump of the rhesus monkey was observed by clinical symptoms，anatomical macroscopic observation and tissue pathological changes by light microscope ，cheek pouch focus of the rhesus monkey has typical features of human squamous cell carcinoma，it was diagnosed squamous cell carcinoma，which would be beneficial to pathologic diagnosis of neoplastic disorder in nonhuman primate animals．

Abstract:Objective To establish an electronic chip data and management system for automatic identification and automatic capture of laboratory monkeys in order to improve the laboratory animal management work． Methods To establish a computer-controlled management system based on the technology of electronic chip implantation and automatic identification to record the data of each monkey，including genetic lineage，physiological data and experimental record， non-touch automatic capture，data reading and writing，electronic record management and remote transmission of data． This working system will considerably reduce the disturbance to experimental monkeys by human factors and stress reaction． Results The application of this system demonstrated that it is practical and easy to use and considerably facilitate the monkey capture and identity recognition during experiment and breeding． Every monkey had the only serial number in its life． This system enabled the working staff to reduce personnel injuries，workload and disturbance to the experimental monkeys． Conclusions A computer-controlled system of automatic identity recognition and automatic capture for laboratory monkeys has been successfully developed． This system provides a scientific，fast and effective computer management mode to create electronic records and managing colony database of monkeys． It can also be adopted to manage large or feral beasts by changing its channels，and has a bright future of application． To our knowledge this is an unique management system and so far is not available elsewhere．

Abstract:Objective To explore the effect of leptin and transforming growth β1 in fibrosis liver． Methods The female Wistar rats were randomly divided into normal control group and four model groups( 1 week ，2 week ，4 week ，6week) ． Carboan tetrachloride was injected for establishing hepatic fibrosis model in model groups． The levels of leptin and transforming growth β1 were determined ． The pathological hepatic changes were observed by HE． The degree of liver fibrosis was observed by stained of sirius red of collagen and the content of hydroxyproline in liver tissues，and the relationship with leptin and transforming growth β1 and FI were amalyzed． The ALT of serum was mensurated with laishi method． Results Compared with control group，the expression of leptin and transforming growth β1 increased significantly in liver fibrosis group ( P ＜ 0. 05 ) ． The expression of leptin and transforming growth β1 in liver tissue were correlated positively with FI in liver fibrosis group． Conclusions The results of the study demonstrate increased leptin and transforming growth β1 levels of liver tissue in liver fibrosis model groups，and activating leptin may up-regulate the expression of transforming growth β1 during experimental hepatic fibrosis．

Abstract:Objective Establishing the method of detecting the simian foamy virus ( SFV ) antibody with Immunofluorescence assay( IFA) ，to provide reference in the the detection of SFV infections in monkeys． Methods Selected SFV sensitive cell BHK-21，take the procedure of one step digestive treatment with trypsin while 50 percent of cell has pathological changes，titrate the cells 40 μL with the concentration of 2 × 107 /mL on the slide． Then it is fixed with acetone． Testing 34 serum with the antigen slide in IFA． Results We established the immunofluorescence assay to test SFV，19 serum was positive，and 15 serum was negative． Conclusion This method can test SFV with high specificity．

Abstract:Objective To explore and establish the method for the skin photosensitization in guinea pigs．Methods TBS was used as the positive control，and the Acetone as the negtive control． The shaved skin of the guinea pigs was irradiated with UV-A radiation of 30 J/ cm2 repeated every 2 days for 3 consencutive days． After 4 weeks of initial sensitization，the skin of the guinea pigs was irradiated with UV-A radiation of 9 J/ cm2． Results Positive ratio of the negtive control 24h，48h and 72h after irridiating was 0，and the positive control was 100% ． Conclusions The skin photosensitization model was established successfully，and the TBS used as the positive control was available．

Abstract:Objective To determine the effect of de-calcification method in making rat-tail section． Methods Rat tail paraffin sections were made by using two different de-calcification methods． Result Both of paraffin sections made by two methods were even and integrated，in which clear structures of rat-trail cells were shown by HE staining． Conclusion The two de-calcification methods are available to make ideal rat-tail paraffin sections in pathological diagnosis．

Abstract:Heart failure is a kind of complex clinical syndrome，it is the end stage of various heart disease who are at high risk for morbidity and mortality． Heart failure severely does harm to human being’S heath，it is quite necessary to gain animal models for heart failure to carry out comprehensive research of its pathogenesis and therapy． This paper reviewed the recent progress of animal models for heart failure and commented on their advantages and disadvantages．