Abstract:Objectives To generate the cardiac-specific human FAM55A transgenic mice，a model for the study of its function and effects on cardiomyopathy． Methods The expression of FAM55A gene in the heart tissues from wild type mice and cTnTR141W transgenic mice were analyzed by western blot． The expression of FAM55A in seven different organs was also detected by western blot． The transgenic vector was constructed by inserting the human FAM55A gene into the downstream of α-MHC promoter． The transgenic mice were created by the method of microinjection． The genotype of transgenic line was identified by PCR and the expression level of the gene was determined by Western blot． The pathologic changes of the heart structure and function were analyzed by echocardiography and microscopy． Results The expression of FAM55A was up-regulated in the mice with DCM． One line of C57BL /6J transgenic mice with high human FAM55A expression was identified from five transgenic founders． Compared with the wild type mice，FAM55A transgenic mice showed increased left ventricular anterior wall during systolic and left ventricular anterior wall during diastole from one month of age to five months of age． The cardiac function of transgenic mice was increased at three months of age． The cardiomyocytes from transgenic mice exhibited hypertrophy and no disarrange under light microscope． Conclusions The expression of FAM55A was up-regulated in the cTnTR141W transgenic mice． The transgenic mice with cardiac-specific expression of the human FAM55A gene were generated and it can be used to crossbreed with the DCM model to investigate the effect of FAM55A gene on the development of cardiomyopathy．

Abstract:Objective To detect the expression of adenovirus E2 promoter binding factor 1 ( E2F-1) and Pituitary tumor transforming gene ( PTTG) in rat prolactinoma and to explore their role in the development of prolactinoma． Methods 40 male Wistar( 120 ～ 150g) rats were randomly divided into two groups: experimental group ( E group，n = 20) : 17β- estradiol stuffed in silicone tube was subcutaneously implanted to induce rat prolactinoma model; the control group ( Cgroup，n = 20) blank silicone tube was subcutaneously implanted． After 10 weeks，all the rats were executed，blood was taken by cardiac puncture，4% paraformaldehyde was intracardially perfused and pituitary tissues were harvested，weighed．Serum PRL levels were detected by ELISA method; Pituitary tissue histopathological changes were observed; both expression of PTTG protein and E2F-1 protein were detected by immunohistochemistry SP method． Results Prolactinoma models were induced successfully as reflected by pituitary weight 、pituitary pathological change and serum PRL level． The expression of PTTG protein and E2F-1 protein in prolactinoma group was obviously higher than those in control group，( P ＜ 0. 01) and PTTG protein expression was positively correlated with E2F-1 protein( γ = 0. 764，P ＜ 0. 01) 。Conclusion PTTG and E2F-1 were co-overexpressed in prolactinoma，indicating their role in the development of prolactinoma．

Abstract:Objective To study the effects of Shanshi Ⅱ mesh to repair abdominal trauma and the tissue reaction after embedding． Compare the effects with other 2 kinds of mesh． Method The rabbits were divided to 3 groups． The model of abdominal hernia was established by surgery and 3 kinds of mesh ( Shanshi Ⅱ，Shanshi PP，chaopu) were planted in 3 groups． The mesh and the tissues that surrounded the mesh were taken out after several weeks． Pathological reaction were observed and shrinking rate of each group were calculated． Results The mesh of Shanshi Ⅱ can improve the adaptability of abdominal muscles． The shrinking rate and the inflammatory reaction is lower comparing with the control group． Conclusion Reducing the content of polypropylene in mesh and adding degradable poly ( glycolide-co-ecaprolactone) ( PGCL) can improve the effects of repair of mesh and restrain inflammatory reaction and fibrosis．

Abstract:Objective To investigate whether the genetically modified somatic cells can survive and express foreign gene for a long time after being transplanted into avian embryo． Methods Chicken DT40 cells as a cell vehicle for delivering foreign protein were transfected with the green fluorescent protein ( GFP) gene，and were introduced into early chicken embryos via blood vessel microinjection at 65 ～ 70 h of incubation at 38. 5 ℃ ． The manipulated eggs were continued to incubate at same condition． The chimerisms of the transplanted DT40 cells were preliminarily observed under fluorescence microscopy at the different stages in the embryos and the hatchlings． Meanwhile，the chimeric positions of the donor DT40 cells and the expression of GFP were further examined by polymerase chain reaction ( PCR ) and immunohistochemistry． Results The results showed that fluorescent-labeled DT40 cells embed in the different organs of the recipients including brain，heart，liver，etc． and can survive before chicken hatch and the GFP gene can be expressed normally． Conclusion Long-term survival of the donor DT40 cells in recipient and normal expression of the GFP gene imply that this approach can be explored for continuous production of target protein in the host chicken，which may provide a basis for avian immune tolerance research and the production of bioreactor．

Abstract:Objective To discuss the effect of cyclosporinA on acellular heterogeneous nerve transplant in Wistar rats． Methods 48 Wistar rats' each side were randomly expeimental side． After facial nerve were unveiled，1 cm defect were produced． All the animals were divided into 4 groups by kinds of graft nerve． Group A: heterogeneous nerve transplant; Group B: heterogeneous nerve transplant with CSA; Group C: acelluler heterogeneous nerve transplant; Group D: acelluler heterogeneous nerve transplant with CSA． 5 and 12 weeks after operation，we carried through the electrophysiology ( latent period，motor nerve conduction speed) and observed the morphological under light and trasmission electronic microscope． Results 5 weeks after operaton，the generation facial nerve of each group were incomplete and the function did not resume． 12 weeks after operaton，the parameters of group C and D were better than group A and B． There were significant difference between group D and other groups on conduction velocity． Conclusion In the experiment of 1 cm facial nerve defect，the CSA can reduce immunoreaction and increase the facial regenarion．

Abstract:Objective To investigate the expression of AT2R and myocardial collagen in heart of different age spontaneously hypertensive rats( SHR) and to explore the role of these factor in the development of hypertension． Methods Male SHR( n = 30) were allocated to five groups by age: 1) one-month-old group( S1) ; 2) two-month-old group( S2) ;3) three-month-old group( S3) ; 4) six-month-old group( S6) ; 5) nine-month-old group( S9) ． Sex and age matched WistarKyoto normotensive rats( WKY) served as controls． Blood pressure was measured． Plasma concentration of angiotensinⅡ ( Ang Ⅱ ) was measured by radioimmunoassay ( RIA ) ． The expression of AT2R in heart was examined by immunohistochemistry and computer image analysis． Collagen of the heart was stained by sirus red to observe the myocardial fibrosis． Results 1． An age-dependent increase of SBP was observed in SHR before six months( P ＜ 0. 05) ，the SBP was higher in the different age SHR than that in the matched WKY( P ＜ 0. 05) . 2． Plasma AngⅡconcentration was higher in S2，S3，S6 and S9 than that in S1( P ＜ 0. 05) ，plasma AngⅡ concentration was higher in S2，S3，S6 and S9 than that in the matched WKY ( P ＜ 0. 05 ) . 3． The quantification of AT2R immunohistochemical staining showed: An age-dependent decrease of the AT2R was observed in heart in SHR( P ＜ 0. 05) ，which was lower in S9 than that in S1( P ＜ 0. 05) ，The AT2R levels were lower in SHR than those in the matched WKY( P ＜ 0. 05) . 4． The content of collagen in the heart of SHR showed: An age-dependent increase of the myocardial fibrosis was observed in SHR ( P ＜ 0. 05 ) ． Conclusions The expression of AT2R protein in heart of SHR is decreased compared with WKY． The content of collagen in the heart of SHR is increased compared with WKY． An age-dependent varies of AT2R protein and the content of collagen was observed in SHR．

Abstract:Objective To explore the role of miR-29c involved in Alzheimers disease． Methods Using highly sensitive microarray-based procedures，we identified microRNA increased in the 3，6，9 month-old APPswe /PSΔE9 doubletransgenic mouse brain，and then validated by real time PCR． Predicted target gene of miRNA were to be chosen though miRNA target databases． We constructed the miR-29c expression vector and transfected it into SH-SY5Y and HEK-293T cell line to validate our hypothesis． We generated the dual-luciferase reporter vectors of APP mRNA 3’UTR and mutant 3’ UTR to identify the binding site of miR-29c by luciferase assay． Results Higherly-expressed miR-29c was measured by microarray and then confirmed by real time PCR in the 3，6，9-month-old APPswe /PSΔE9 double-transgenic mouse brain ．APP is predicted to be downregulated by miR-29c by miRBase databases． We demonstrated APP protein decreased in overexpressed miR-29c SH-SY5Y cell line by Western blot． Then we confirmed miR-29c can decreased APP protein expression by cotransfecting miR-29c expression vector with APP cDNA vector( involved 3’UTR in full length) to HEK- 293T cell lines． In particular，we had not detected the target site of miR-29c in the APP mRNA 3’UTR by luciferase assay in HEK-293T cell line． Conclusions miR-29c can downregulate APP expression in vitro，but the target site may be not in the APP mRNA 3’UTR．

Abstract:Objective To compare the sensitivity of methods for detecting H5N1 influenza virus． Methods The H5N1 influenza virus was amplified and quantified by plaque technology． Then the virus was diluted to compare the minimal titer using methods of cell inoculation，hemagglutinin，RT-PCR and Real-time PCR． Results The minimal titer detected using the methods of cell inoculation，hemagglutinin，RT-PCR and Real-time PCR were 10 PFU /mL，10 PFU /mL，103 PFU /mL and 3. 52 × 105 PFU /mL seperately． Conclusions We found that the methods of cell inoculation and real-time RT-PCR were the most sensitive methods; while the hemagglutinin method was timesaving，it showed low sensitivity which need to be identified in future; RT-PCR method was timesaving，in addition，it also showed high sensitivity．

Abstract:Objective To establish a new method for rat kidney without traumatic local vascular drugs ，and after the treatment of its survival rate and traumatic carries on the observation． Methods Wistar rats，PE10 catheter through from the left common carotid artery- the descending aorta- the thoracic aorta- the abdominal aorta，arrived in renal artery directly to medicine． Statistical its survival rate and dosing renal arterial line pathology observation． Results Rats survival rate can reach 87. 5% ，and wont cause renal arterial thrombosis or vascular injury． Conclusions The method can be successfully for rats renal artery without traumatic local blood vessels to medicine，also can be extended for abdominal some of the other organs or the hind legs．

Abstract:Objective To establish the Influenza H5N1 influenza virus infection ferrets animal model． Methods Groups of three 4-to 6-month -old ferrets were inoculated intranasally with 103 and 104 TCID50 of H5N1 A /Vietnam /1203 /2004 in a volume of 500uL． Ferrets were monitored daily for clinical signs of influenza infection，and body temperatures were recorded once daily． Every day take nasal turbinates for RT-PCR． On day 5 postinoculation，ferrets were euthanized and trachea，lung，heart，liver，slpeen，kidney，small intestine，and brain tissue were harvested，and examined by light microscopy． Results For each titer virus tested，in infected ferrets，we observed fever，weight loss，nasal rattling，sneezing and died． Virus was detected in the nasal turbinates and heart，liver，slpeen，kidney，small intestine，and brain from all of the iinfected ferrets． Of the ferrets inoculated with H5N1 virus，there show focal severe pneumonia in lung．Conclusions In clinical signs，virus detection，and histological changes were observed ferrets were infected H5N1 influenza virus，which 104 TCID50 titer was a more appropriate to establish flu animal models．

Abstract:Objective To establish the Influenza H5N1 influenza virus infection ferrets animal model． Methods Groups of three 4-to 6-month -old ferrets were inoculated intranasally with 103 and 104 TCID50 of H5N1 A /Vietnam /1203 /2004 in a volume of 500uL． Ferrets were monitored daily for clinical signs of influenza infection，and body temperatures were recorded once daily． Every day take nasal turbinates for RT-PCR． On day 5 postinoculation，ferrets were euthanized and trachea，lung，heart，liver，slpeen，kidney，small intestine，and brain tissue were harvested，and examined by light microscopy． Results For each titer virus tested，in infected ferrets，we observed fever，weight loss，nasal rattling，sneezing and died． Virus was detected in the nasal turbinates and heart，liver，slpeen，kidney，small intestine，and brain from all of the iinfected ferrets． Of the ferrets inoculated with H5N1 virus，there show focal severe pneumonia in lung．Conclusions In clinical signs，virus detection，and histological changes were observed ferrets were infected H5N1 influenza virus，which 104 TCID50 titer was a more appropriate to establish flu animal models．

Abstract:Objective To research the clinical diagnosis of lung disease in rhesus monkey with the observation of lung CT tomography of the adult laboratorial rhesus monkey，to establish the maps of CT tomography of the healthy adult rhesus monkey，to provide the basic image data of CT technology in the applications of anatomy，clinical diagnosis and scientific experiments of rhesus monkey． Methods 5 male and 5 female health adult monkeys aged 5 ～ 10 years were selected for this study of lung CT tomography after being checked by palpation，percussion，auscultation and by examination of body temperature，respiratory rate，heart rate，breathing motion and blood parameters． After general anesthesia，the monkeys were placed on the table-board of CT in supine position and were scanned to get the image of lung CT tomography．The main structure of every level in scanned images were labeled，such as lobe，trachea，arteries and veins，etc． Results ( 1) 13 anatomical images of lung CT tomography are obtained． ( 2) In the image of CT tomography，lung，trachea，large blood vessels are all in focus． Lungs are composed of the right part and the left part，the left lung is divided into upper lobe，middle lobe and lower lobe． Right lung is divided into upper lobe，middle lobe，lower lobe and azygos lobe． Left main bronchus，right main bronchus，bronchial and vascular are very obvious in different scanned images． ( 3) Interfaces of small blood vessels，small nerves and muscle tissues are not clearly defined on the CT scanned images． Conclusions ( 1) The images of lung CT tomography of the healthy adult rhesus monkey show that all the lung lobes are absolutely clear，lung transmittance is good，and there are no any shadows． ( 2) Obtained the anatomy CT tomography image data of healthy adult rhesus monkey． It can provide a new safe，convenient and accurate basis for diagnosis of monkey lung disease． It also established the background Information data of CT tomography anatomy of the healthy adult rhesus monkey．

Abstract:Objective Preparation and characterizing of porcine parvovirus ( PPV) hybridomas cells and their monoclonal antibody． Methods Two hybridomas cells were obtained by conventional methods． ． The hybridomas cells were analysed and identified by chromosome analysis，indirect ELISA，the immunoperoxidase monolayer assay ( IPMA) and indirect immunofluorescence ( IFA) methods． Results Two hybridomas cells were obtained and named 2H9 and 1F9 respectively． The chromosome numbers were between 90 to 110. Titration of the hybridomas culture supernatant were 1∶ 1× 104，and titration of ascitic fluid were 1 ∶ 1 × 107，their subclasses were IgG1，IgM，belonging to kappa chain，and without cross-reaction with PRRSV，CSFV，PRV，PCV-1，PCV2 and JEV． Furthermore，the specific reaction of antibodies and PPV in PK-15 cells was confirmed IPMA and IFA． Conclusion Two anti-PPV hybridomas cells were acquired and the characterizations of two monoclonal antibodies were confirmed ．

Abstract:Objective Use the proved Non-human Primate Neurological deficit scale to evaluate the effect of Intracerebral Hemorrhage model of Cynomolgus macaques Treatment with Human Bone Marrow Mesenehymal Stem Cells． Methods 12 adult male Cynomolgus monkeys were used in this study． The Intracerebral Hemorrhage model were made by using the autologous anticoagulated femoral artery blood injection． 12 monkeys were divided into three groups one week after the surgery． The control group consisted of four animals receiving physiological saline in the volume of 250μl 3 mm away from the outside edge of the infarcts． The high-dose and low-dose group receiving Human Mesenchymal Stem Cell( hMSC) with the cell number of 5 × 106 and 1 × 106 at the same place and volume with the control group． Use the Non-human Primate Neurological deficit scale and / or proved to evaluate the effect of the stem cell at the 6h，1d，3d，1week after surgery and 1，3 day and 1，2，3，4 weeks after transplantation． Result Compared with the group，the score of the dose-group animals much slower than that in the control group after transplantation ( p ＜ 0． 05) ． There is no significant difference between high-dose and low-dose group． Conclusion The proved Non-human Primate neurological deficit scale is more objectively and accurately than the original one and can be used to measure neuronal loss in vivo and to evaluate the effects of therapeutic strategies involving neural or stem cell transplantation．

Abstract:Mouse is the most popular laboratory animal in medical research． As an animal model，mouse has been used for human cancer research for over one century，a large number of genetic variations in mice can be used as reference for the study of human cancer． Over the past 20 years，genetically engineered mouse( GEM) ，including transgenic mouse，knock-out mouse，have been cultured，thus，paved a way to deepen our understanding of cancer diagnosis，innovative therapeutic，and prevention． In this review paper，we mainly introduce spontaneous and inducible cancer mouse models and GEM models． In addition，some recent strategies and accomplishments for producing complicated cancer models in the mouse as well as the limitation of mouse model are discussed．

Abstract:Laboratory animal center is one of the technology platform for the hospital scientific research ． How to use the limited resources for improving the service level，which is essential to resolve the question as a manager in laboratory animal center ． In this article，combining the standardization construction and management of the laboratory animal center of PLA Institute of Stomatological Research，the methods in operating efficiency and servicing quality of the laboratory animal center were discussed ．

Abstract:The studies on emerging viral diseases are restrained by a “bottle neck”: no competent animal model available． Models based on nonhuman primates have similar clinical manifestations to human diseases; however，it is uneconomic to construct such models． Furthermore，there is a controversy about protection of animal rights． Models based on rodents are often incompetent due to their low susceptibility for those viruses． This brief review focuses on the current studies on relations among nutrition，immunity and susceptibility to give insights in overcoming conundrums．

Abstract:Objective To develop an instrument that detects the behaviours of food and thrink of animals during pharmacological and bromatological experiments． Methods Consists of infrared sensors， Pressure sensors and Photoelectric sensors were used for signal conversion． Reduction motors were used for channels door control． A 51-Series microcontroller was used to control the experimental setup and record the results． Results We have developed a microcontroller-based instrument that can automatically detects the behaviours of food and thrink of animals during pharmacological and bromatological experiments． Conclusion Our experimental results with 30 mice indicate that the instrument developed provides an innovative tool for detecting the behaviours of food and thrink of animals．