Abstract:目的 构建全长与成熟形式IL-37b 的真核表达载体pEGFP N1/ IL-37b,检测二者在RAW 264.7 细胞中的表达情况?方法 以含IL-37b 全长编码区基因的质粒pUBC/ IL-37b 为模板,构建能够表达全长与成熟形式IL-37b 的真核表达载体?将构建的重组质粒pEGFP N1/ IL-37b 转染到RAW 264.7 细胞中,通过western blot 和共聚焦显微镜检测全长与成熟形式的IL-37b 的表达情况,并通过real-time PCR 检测全长与成熟形式IL-37b 对LPS 诱导的IL-6 表达的抑制作用?结果 构建的pEGFP N1/ IL-37b 转染后能够在细胞中表达全长与成熟形式的IL-37b,并且能够抑制LPS 诱导的IL-6 的表达?结论 成功构建了全长与成熟形式IL-37b 的真核表达载体,为进一步研究IL-37b 的炎症抑制作用与机制打好基础?

Abstract:Objective To explore the effects of Toddalia asiatica extract (TAE) on inflammatory cytokines in rats with myocardial ischemia and hyperlipidemia. Methods The early changes of hyperlipidemia were caused by feeding high fat diet for 4 weeks. Fifty male SD rats were randomly divided into 5 groups: the control group, model group, simvastatin group (3.5 mg/ kg), high-dose TAE group (280 mg/ kg) and low-dose TAE group (70 mg/ kg). At the end of the 4-weeks treatment, isoprenaline hydrochloride (Iso) was subcutaneous injected once a day for consecutive three days. The electrocardiogram (EKG),the heart,liver and fat indexes, the serum levels of TC, TG, HDL, LDL, TNF-α, INF-γ, IL-6 and IL-10, and the pathological changes of myocardium were examined. Results TAE significantly improved the Isoinduced EKG changes in rats, reduced the heart, liver and fat indexes,and decreased the levels of TC, TG, LDL, TNF-α, INF-γ and IL-6 and increased the contents of HDL and IL-10 (P < 0.05 for all), and improved the pathological damages of myocardium. Conclusions The Miao nationality medicine Toddalia asiatica extract may play an important protective role in cardiovascular diseases by regulating the balance of antiinflammatory and proinflammatory cytokines.

Abstract:Objective The effect of different doses of ethylnitrosourea (ENU) and cyclophosphamide (CP) on the loss rate of CD59 on peripheral blood erythrocytes was explored to optimize the detection method of Pig-a gene mutation. Methods According to the weight and loss rate of CD59 on peripheral blood erythrocytes, rats were divided into 4 groups: the control group, CP 40 mg/ kg group, ENU 10 mg/ kg group and ENU 40 mg/ kg group (n =6). The control group was injected i. p. with PBS, other groups were injected i. p. with corresponding solutions. The body weight of rats on days 0, 7, 14, 21, 28, 42 and 56 were recorded. At the same time, blood samples were collected and incubated with antibodies, and the loss rate of RBC ^CD59- was detected by flow cytometry. Results Compared with the control group, at different time points, the body weight and weight gain of ENU 10 mg/ kg group and ENU 40 mg/ kg group had no statistically significant difference (P > 0.05), while those in the CP 40 mg/ kg group were significantly decreased (P <0.05). The loss rate of RBC ^CD59- was significantly increased in the CP 40 mg/ kg group at 28, 42 and 56 days, ENU 10 mg/ kg group at 42 and 56 days, and ENU 40 mg/ kg group at 7, 14, 21, 28, 42 and 56 days, (P < 0.05). The results showed a dose-response relationship. Conclusions Under the conditions of this Pig-a mutation detection method , ENU is superior to CP on raising loss rate of RBC ^CD59- , ENU 40 mg/ kg is better than 10 mg/ kg, and 28 days is suitable as the test period.

Abstract:Objective To study the effect of autologous periosteum on the healing of tendon-bone interface in rabbit rotator cuff tear. Methods Supraspinatus tenotomy was performed on one side shoulder in 60 New Zealand white rabbits to establish the model of rotator cuff tear. The rabbits were randomly divided into two groups: the study group (used autologous periosteum to promote the suture fixation) and control group (simple suture fixation). The rabbits were sacrificed at 4, 8 and 12 weeks postoperatively with twenty rabbits sacrificed each time. Tissue samples of the tendon-bone interface was taken for histological examination and biomechanical test was performed to assess the strength of tendon-bone junction. Results At 4 weeks the study group showed extensive inflammatory cell infiltration, and a small amount of chondrocytes and extracellular matrix. At 8 weeks, the study group showed a large amount of immature chondrocytes arranged rather regularly. At 12 weeks, the tendon bone junction appeared similar to normal. In the study group, both the tendon-bone connection and the arrangement of chondrocytes were significantly better than the control group. The result of biomechanical testing showed that the highest tendon load in the study group was significantly higher than the control group (P < 0.05). Conclusions The use of autologous periosteum as a patch to strengthen the repair of the rotator cuff tear can effectively promote the healing of tendon bone interface, shorten the rotator cuff healing time and has good biological properties. This method provides an experimental basis for clinical rotator cuff repair surgery.

Abstract:Objective BALB/ c mutant curly mice and normal BALB/ c mice were genetically detected by microsatellite DNA marker analysis to detect the differential microsatellite loci between BALB/ c mutant curly mice and normal mice. Methods 38 microsatellite DNA loci were selected and their variation in the BALB/ c mutant curly mice, BALB/ c mutant hairless mice and normal BALB/ c mice were detected by multiplex fluorescence PCR and STR scanning genotyping. Results There were 27 the same microsatellite loci between the 38 microsatellite loci in BALB/ c mutant curly mice and normal mice, and there were 11 differential loci, with a mutation rate of 28. 9% (11/38). There were 30 the same sites between BABL/ c mutant hairless mice and normal mice, and there were 8 different loci, with a mutation rate of 21.1% (8/38). There were also 12 differential loci between BABL/ c mutant curly mice and hairless mice. Conclusions BALB/ c mutant curly mice have a higher mutation rate and are significantly higher than those of hairless mice, demonstrating that the mutations in curly mice and hairless mice are two completely different mutations. These results provide reliable theoretical data for the future study and development of BALB/ c mutant curly mice.

Abstract:Objective The ammonia level in air and heavy metals in drinking water were explored in a cynomolgus monkey feedlot. Methods Air ammonia from different communities and feeding patterns of cynomolgus monkeys were collected at three time-points per day and determined by Nessler’ s reagent spectrophotometry. Inductively coupled plasma mass spectrometry was applied to detect the level of heavy metals in drinking water from 5 different sampling sites in the feedlot, incluing the outlet of underground water, the water tank, both monkey cages equipped with PVC or iron pipes and sewage lagoon, respectively. Results Air ammonia levels in quarantine inspection flock cages (0.59 ± 0.03 mg/ m3 )were significantly higher than in the cages of both reproductive flock (0.34±0.03 mg/ m3 ) and sale flock (0.27±0.04 mg/ m3 ). The ammonia level in air in different feeding patterns ranks as following: cage rearing of quarantine inspection flock (0.59± 0.03 mg/ m3 ) > cage rearing of reproductive flock (0.48±0.02 mg/ m3 ) > captive bleeding of sale flock (0.30±0.02 mg/m3 ) > cage rearing of sale flock (0.25±0.01 mg/ m3 ) > captive bleeding of reproductive flock (0.22±0.02 mg/ m3 ). The air ammonia concentrations of both former flocks were statistically higher than the latter three flocks. The highest air ammonia level among different flocks and feeding patterns occurred in the morning, before waste discharge clean-up. The iron concentration in drinking water in the cages equipped with iron pipes was higher than Chinese drinking water standard. Conclusions The air ammonia level was lower than the Chinese air quality standard. The iron concentration in drinking water in the cages equipped with iron pipes was higher than the Chinese drinking water standard.

Abstract:Objective To Establish a loop-mediated isothermal amplification (LAMP) assay for detection of Salmonella in fecal samples of tree shrews, and report the result of preliminary application of this method. Methods LAMP primers were designed and synthesized according to the conserved sequence of Salmonella specific gene invA (invasive protein gene A). To optimize the reaction time and temperature by setting 10 reaction times (24 to 42 min) and temperature (57℃ to 66℃) and tested its specificity and sensitivity. At the same time, a conventional PCR test was performed to verify and compare with the LAMP assay. 91 fecal samples of wild-derived tree shrews were detected by the LAMP assay. Results The experimental condition was confirmed as 62℃ and 34 min. The sensitivity of Salmonella was 3.36 ×101 CFU/ mL, which was 10 to 100 times higher than that of conventional PCR assay. In 10 kinds of intestinal bacteria for LAMP amplification, Salmonella enteritidis and Salmonella paratyphi B were positive, the others were negative. Among the 91 samples of tree shrew fecal samples detected by the LAMP assay, the positive detection rate was 20.88%. The LAMP assay can be completed within 40 min, the result can be observed and judged visually by color changes. Conclusions The LAMP assay established in this study is convenient, rapid, sensitive and specific. It can be used as a rapid measure for large?scale detection of Salmonella in feces of tree shrews.

Abstract:A laboratory animal expert consultation system provides an interactive platform for industry experts and users. Based on intelligent mobile terminal used as the application supporter, the platform combines the internet with laboratory animal to share the experts’ knowledge, breaks through temporal and spatial restrictions, and resolves other problems such as poor timeliness of inquire service, knowledge data staticization, and irrational exploitation of internet to gain prompt inquire service. The platform would meet the needs of industry users for real?time aids and bridge the gap between industry experts and users. Thus, the transfer of expert’ knowledge is realized in pace with the increasing knowledge resource of this platform to accelerate the transformation of theoretical knowledge into the industry. Our study focuses on the system characteristics, the main frame structure, the design of function module and the system implementation.

Abstract:Long non-coding RNA (LncRNA) has attracted increasing attention over the recent years. It is closely related to the occurrence and development of human diseases based on numerous academic researches. Analysis of the biological function of LncRNA in depth may lead to great breakthroughs in clarifying pathogenesis of many diseases and provide possible targets for intervention. Recent studies have revealed the importance of LncRNA in the progression of heart failure (HF), the final stage of many cardiovascular diseases. This review summarized the new findings of LncRNA in prognosis and treatment of HF.

Abstract:The proliferation of tumor cells is regulated by a complex array of signaling pathways, among these signaling pathways, the programmed cell death. Autophagy and apoptosis are two types of programmed death. There are significant differences in their morphological and functional features, but they also have many links. Both apoptosis and autophagy are involved in activation, expression and regulation of a series of genes. By reviewing the research progress in recent years, this article will discuss the cellular regulation and molecular mechanisms of their related genes. Through summarizing the relationship between autophagy and apoptosis, it aims to get a better understanding of the mechanisms of autophagy and apoptosis in tumor progression, and looking at the perspectives for studies on the autophagy and apoptosis in tumor treatment.

Abstract:Breast cancer is the most common spontaneous malignancy in women, causing a serious threat to women’s health. Experimental animal models have been important research tools in bringing insights into the related mechanisms of breast cancer, as well as in delivering improved therapies for the disease. To reveal the pathogenesis of various breast cancers and facilitate the development of new drugs, ideal animal models of breast cancer should share some common tumor molecular characteristics and biological behavior with that of human breast cancer. In this review, five types of experimental animal breast cancer models were included, which may provide a basis for research on diagnosis and treatment of human breast cancer.

Abstract:Objective A state quo survey of laboratory animal resource in Guangdong province is performed to provide reference data for government management decision-making and market assessment of laboratory animals. Methods We used questionnaires focusing on the laboratory animal facilities with authorization by Guangdong Province Government, which mainly included the production and use of laboratory animals, the qualification of employees and the facilities space. Results The total production and use of laboratory animals (except for the eggs) had been increasing in the last four years. 1.57 million laboratory animals were produced and 0.754 million laboratory animals were used in 2016. There were 2352 employees, roughly the same as in 2015. The facilities space for breeding was 121008 m² , and for animal experiment was 73470 m² , which were rising in the past three years. Conclusions In order to reinforce the industry development of laboratory animals in Guangdong province, some suggestions were given in our study, such as facilitating the application of superiority resource including non-human primate and aquatic laboratory animals, supporting the standardization production of several scarce mice and rats, improving relevant employees’ overall level and constructing laboratory animal facilities sharing platform.

Abstract:Laboratory red crucian carp is a native fish in China. It has been an established inbred fish strain, the laboratory red crucian carp C1HD strain. Based on the standardization research and verification test of laboratory red crucian carp, the local standard of Hunan Province, the called “Laboratory Fish Genetic Quality Control of Laboratory Red Crucian Carp C1HD Strain”, has been established. This standard includes preface, scope, normative reference documents, terms and definitions, major biological characteristics, mating and breeding method , breeding environment,genetic quality testing and appendix A, B, C and so on.