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ZHANG Xiuqing , LIU Juan , LIU Bin , LIU Ping
2022, 32(11):1-11. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 001
Abstract: Objective To investigate the role and potential mechanism of long non-coding RNA sequence similarity family 83 member A-antisense ribonucleic acid 1 ( lncRNA FAM83A-AS1) in breast cancer (BC). Methods The expression of FAM83A in BC tissue was detected by immunohistochemistry, and lncRNA FAM83A-AS1 and FAM83A mRNA in BC tissues/ cells was detected by real-time fluorescent quantitative PCR (qRT-PCR). Pearson method was employed to test the correlation between lncRNA FAM83A-AS1 and FAM83A mRNA expression levels in BC tissues. Cell transfection silencing of MDA-MB-231 was used to study gene overexpression. The expression levels of lncRNA FAM83AAS1 and FAM83A mRNA in MDA-MB-231 cells were detected by qRT-PCR. MDA-MB-231 cell proliferation activity was detected via the CCK-8 method. MDA-MB-231 cell migration and invasion abilities were detected by Transwell experiment. The protein expression of FAM83A, ERK1 / 2 and its phosphorylated protein Ki-67, E-cadherin and matrix metalloproteinase 9 in MDA-MB-231 cells was detected by Western blot. The subcellular distribution of lncRNA FAM83AAS1 was detected by nucleocytoplasmic separation experiments, and the interaction between lncRNA FAM83A-AS1 and FAM83A was verified by RNA pull-down, RNA immunoprecipitation (RIP) and qRT-PCR assays. Nude mice were used in a tumorigenesis experiment to detect the effect of lncRNA FAM83A-AS1 silencing on the growth of MDA-MB-231 cells in vivo. We employed immunohistochemistry staining to detect the expression of Ki-67 and FAM83A in tumors. Results The expression of lncRNA FAM83A-AS1 and FAM83A mRNA in BC tissues and cells was significantly up-regulated ( P<0. 05). Pearson analysis showed that the expression levels of lncRNA FAM83A-AS1 and FAM83A mRNA in BC tissues were positively correlated (r= 0. 885, P<0. 05). LncRNA FAM83A-AS1 silencing reduced the proliferation of MDA-MB-231 cells; inhibited the migration and invasion of MDA-MB-231 cells; promoted E-cadherin expression; inhibited FAM38A, Ki-67 and matrix metalloproteinase 9 expression and ERK1 / 2 activation; and inhibited the growth of transplanted tumors in nude mice (P<0. 05). Up-regulating the expression of FAM83A significantly reduced the inhibitory effects of lncRNA FAM83A-AS1-silencing on proliferation, migration, and invasion by MDA-MB-231 cells (P< 0. 05). LncRNA FAM83A-AS1 is present in both the nucleus and cytoplasm and can bind to FAM83A through the RNA-binding protein FBL to increase the expression of FAM83A. Conclusions LncRNA FAM83A-AS1 can promote proliferation,migration, and invasion by MDA-MB-231 cells by up-regulating FAM83A expression.
ZHANG Na , LIU Xuefang , FENG Yanyan , GUO Fengyue , FENG Suxiang
2022, 32(11):12-17. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 002
Abstract: Objective To study the tumorigenesis and metastasis of Lewis lung cancer derived from lung metastases in C57BL/ 6 mice. Methods Lewis lung cancer tumor-bearing mice with lung metastasis were dissected, and lung metastases were isolated to prepare a cell suspension, which was inoculated subcutaneously into the axilla of the right forelimbs of C57BL/ 6 mice. A mouse model of Lewis lung cancer derived from lung metastases was established and designated as the lung tumor group. Subcutaneously transplanted tumor passaged mice were used as the subcutaneous group. The tumor diameter of mice was measured periodically, the tumor volume was calculated, and a survival curve was drawn. Anatomical observation was performed on lung and liver lesions, and HE staining was used to detect pathological changes in the lungs and liver. The ultrastructure of tumor cells was observed by transmission electron microscopy. Results The tumor volume of the lung tumor group was less than that of the subcutaneous group(P>0. 05). The survival rate of the lung tumor group was higher than that of the subcutaneous group during the observation period. Anatomic observation showed the lung and liver metastasis rates to be 37. 5% and 25% in the lung tumor group and 20% and no liver metostasis in the subcutaneous group, respectively. In HE staining, lung and liver metastases in the lung tumor group were large,darkly stained, approximately round in appearance, and demarcation from surrounding tissues was obvious. Lung metastases in subcutaneous group were small, and no typical liver metastases were seen. Tumor cells in the metastases showed typical bizarre nuclei and pathological mitosis under transmission electron microscopy. Conclusions Compared with subcutaneous tumor mice, lung tumor mice showed slower tumor growth, a higher survival rate within the same observation period, and stronger distant metastatic properties.
SHEN Baoyu , REN Yanming , YANG Genmeng , YU Hao , DONG Wenjuan , HONG Shijun , ZHANG Ruilin
2022, 32(11):18-25. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 003
Abstract: Objective This work was aimed at investigating the intervention effects of cannabidiol (CBD) on the monoamine neurotransmitter in a rat model of methamphetamine ( METH) dependence, and we revealed the potential mechanism underlying the therapeutic effects of CBD. Our research will benefit the treatment of drug dependence in humans. Methods We constructed an animal model based on METH ( 2 mg / kg) exposure and the conditioned place preference procedure. We dissected the nucleus accumbens (NAc), frontal cortex(FC), ventral tegmental area (VTA),caudate putamen(CPu), and hippocampus(Hip). The dopamine, 5-hydroxytryptamine, and norepinephrine contents were separated using high-performance liquid chromatography and quantified with mass spectrometry. Results Repeated METH (2 mg / kg) exposure significantly increased dopamine content and decreased 5-hydroxytryptamine content in the NAc, frontal cortex, VTA, caudate putamen, and hippocampus but increased norepinephrine content in the NAc and VTA of rats. Pretreatment of rats with CBD (10, 20, 40, 80 mg / kg) dose-dependently reduced the impact of METH on these monoamine neurotransmitters. Conclusions CBD may attenuate the reward effect of METH by maintaining the homeostasis of monoamine neurotransmitters in the reward-related brain regions of rats.
ZHANG Bosheng , GUO Pingping , YE Chenyu , ZHANG Haiying
2022, 32(11):26-33. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 004
Abstract: Objective In vitro cell experiments were conducted to study the proliferation-inhibition and apoptosis effects of five species of Ferula from Xinjiang and several polar regions on human colon cancer HCT 116 cells, and an in vivo study examined the effects of five species of Ferula from Xinjiang on colon cancer CT-26 mice. Methods Human colon cancer HCT 116 cells were treated with five species of Ferula from Xinjiang and several polar regions. The optical density absorbance was measured by MTT method , the proliferation-inhibiting rate was calculated, and the cell apoptosis rate was measured by flow cytometry. A BALB/ c colon cancer CT-26 mice tumor model was established and randomly divided into a normal, model, cisplatin and Ferula species high-dose and low-dose groups. On the 11th day, the mice were sacrificed, the tumor, spleen, kidneys, and thymus were removed, and the organ index and tumor inhibition rate of each group were calculated. Results For HCT 116 cells, the inhibitory IC50 values of Ferula ferulaeoides, F. songorica, and F. sinkiangensis were 26. 06, 28. 31 and 42. 27 μg / mL, respectively, while the inhibitory effect of F. krylovii was weak, with an IC50 of 131. 61 μg / mL. F. conocaula had the weakest inhibitory effect, with an IC50 of 261. 77 μg / mL. Petroleum ether, methylene chloride, and ethyl acetate extracts of F. songorica had obvious inhibitory effect on HCT 116 cells, but the water-saturated n-butanol extract had only a weak inhibitory effect, and the water extract had almost no inhibitory effect. Among the five species of Ferula found in Xinjiang, F. songorica had the best apoptotic effect on HCT 116 cells. Compared with the normal group, colon cancer CT-26 mice treated with Ferula tended to have decreased spleen, kidney, and thymus weights and organ index, which is consistent with the positive-control cisplatin drug group. In terms of tumor inhibition effect, the tumor inhibition rates of F. songorica high- and low-dose groups were 61. 73% and 67. 40%, respectively. Conclusions The anti-tumor effect of F. songorica is promising and worthy of further study.
WANG Xianzhen , LI Yi , WU Xiaowei , CUI Qiang
2022, 32(11):34-42,56. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 005
Abstract: Objective To investigate the effect of exosomes ( Exos) derived from rat epidermal stem cells (EPSC) overexpressing microRNA-26a (miR-26a) on wound healing in rats with deep second-degree burns. Methods Rat EPSCs were cultured in vitro and transfected with lentiviral particles carrying miR-26a-green fluorescent protein (GFP) and negative control (NC-GFP) to up-regulate the expression of miR-26a. Exos were separated from untransfected, NCGFP-transfected, NC-GFP-transfected and miR-26a-GFP-transfected EPSCs. Transmission electron microscopy and nanoparticle tracking analysis were performed to observe the shape and size of the Exos. Western blot was performed to measure the expression of Exo surface markers CD9, CD63 and CD81. Real-time fluorescent quantitative PCR was performed to measure the expression of miR-26a in EPSC and EPSC-Exos, and the CCK-8 method was applied to measure the proliferation activity of EPSC. A tubule formation experiment was performed to evaluate the effect of miR-26a-EPSCExos on angiogenesis. A rat model of deep second-degree burns was established and separated into control, NC-EPSCExos, and miR-26a-EPSC-Exos groups, with 30 rats in each group. The NC-EPSC-Exos and miR-26a-EPSC-Exos groups ,were given corresponding Exos intervention, and the control group was given an equal volume of PBS once a week for 3 weeks. On the 7th, 14th and 21st days after rats were burnt, the burn wound conditions of the rats in each group were observed and the wound healing rate was calculated. HE stainning was performed to observe histopathological changes of the wounds, and histological scores were obtained. An immunohistochemical method was employed to measure the positive expression of CD31 on the wound and calculate the microvessel density. Results miR-26a transfection significantly increased the expression of miR-26a in EPSC and its Exos and promoted the proliferation of EPSCs ( P< 0. 05 ). Transmission electron microscopy and nanoparticle tracking analysis result showed that Exos were spherical, with a diameter ranging from 40 nm to 150 nm, and positive for CD9, CD63, and CD81. The tubule formation experiments showed that miR-26a-EPSC-Exos was able to significantly increase the tube length and promote endothelial cell angiogenesis ( P<0. 05). The result of the in vivo experiments showed that miR-26a-EPSC-Exos significantly accelerated wound healing and repair in rats with deep second-degree burns and increased the histological score and microvessel density ( P< 0. 05). Conclusions EPSC-Exos overexpressing miR-26a can promote wound healing in rats with deep second-degree burns.
WANG Xiaohong , SUN Kai , PIAO Li , XU Zhaoning , YU Xiuli , LIN Jianan , ZHOU Li , XIA Meihui
2022, 32(11):43-48. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 006
Abstract: Objective To investigate the effect of resveratrol on the repair of hypoxic brain injury through the HIF-1α/ BNIP3-mediated autophagy signaling pathway in mice. Methods A mouse model of intermittent hypoxia was established using the CQY-1 small animal hypoxia detection system. The hypoxia tolerance time, respiratory rate, and heart rate changes of the mice were detected. Histopathological staining was used to detect morphological changes to the mice brain. Western blot was used to detect the protein expression levels of HIF-1α, P53, BNIP3, Beclin 1, LC3 and P62 in mice brain tissue. Results Compared with the findings in the hypoxia group, resveratrol prolonged the hypoxia tolerance time; increased the respiratory rate and reduced the heart rate of hypoxic mice; alleviate brain tissue damage in the hippocampus; down-regulated HIF-1α, P53, Beclin 1 and LC3 protein expression; and up-regulated P62 protein expression. Conclusions Resveratrol down-regulates HIF-1α/ BNIP3-mediated autophagy signaling pathway gene expression to promote the repair of hypoxic brain injury in mice.
ZHOU Gaofeng , XIAO Jing , ZHOU Jia , LIU Junxiu , LYU Guangfu , WANG Yuchen , LIN He , HUANG Xiaowei
2022, 32(11):49-56. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 007
Abstract: Objective To observe the protective effects of velvet antler peptide (VAP) on myocardial infarction (MI) ischemia and fibrosis injury in rats after coronary artery ligation and the mechanism of TGF-β/ Smad signaling pathway mediation by VAP. Methods 60 SPF male Wistar rats were randomly divided into six groups: Sham operation ;MI; positive drug control ( captopril, 30 mg / kg); low-, medium-, and high-dose ( 100, 200, and 300 mg / kg) VAP groups. The left anterior descending limb of the coronary artery was ligated to provide a model of myocardial injury in rats.Three days after the model was made, the rats were orally administered with 1 mL/ d VAP for 28 days. Two hours after the last administration, we collected blood aseptically from the abdominal aorta of anesthetized rats and centrifuged the samples to obtain VAP serum. Electrocardiogram was used to analyze the degree of myocardial injury. HE staining was used to observe pathological changes in the myocardial tissue of rats. Serum creatine kinase isoenzyme ( CK-MB) and cardiac troponin (cTn) levels were measured by enzyme linked immunosorbent assay ELISA. The levels of TGF-β1, Smad2 / 3, pSmad2 / 3, Smad4, Smad7, Collagen I and Collagen Ⅲ proteins in myocardial tissue were detected by Western blot. Results HE staining in the MI group showed that myocardial fibers group were disordered; transverse stripes had disappeared; cells were swollen, cracked, and necrotic; and nuclei were deformed and displaced compared with the findings in the Sham group. The pathological morphology of myocardium in KTPL group and VAP group was significantly improved compared with that in the MI group. ELISA showed significantly higher CK-MB, cTnT (cardiac troponin T), and cTnI (cardiac troponin I) levels in the MI group compared with the findings in the Sham group (P<0. 01). There were significantly lower CK-MB, cTnT, and cTnI contents in the myocardial tissue of positive drug control group KTPL and VAP groups compared with the findings in the MI group (P<0. 01). The Western blot showed that myocardial injury was caused by the up-regulation of TGF-β1, Smad2 / 3, p-Smad2 / 3, Smad4, Collagen I and Collagen Ⅲ and down-regulation of Smad7 protein expression in the MI group (P<0. 01). Compared with the findings in the Sham group. The expression of TGF-β1, Smad2 / 3, p-Smad2 / 3, Smad4, Collagen I and Collagen Ⅲ were significantly down-regulated and Smad7 was up-regulated in the KTPL group and VAP group, which improved fibrosis (P< 0. 01). Conclusions MI rats activate TGF-β/ Smad signal transduction, and captopril and VAP inhibit TGF-β1 and Smad proteins to improve myocardial fibrosis after MI. VAP may protect against myocardial ischemia and fibrosis after MI by regulating the TGF-β/ Smad signaling pathway.
WANG Jiangbo , LIANG Hao , LU Yunping
2022, 32(11):57-67. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 008
Abstract: Objective MicroRNAs are involved in the occurrence and development of many cancers, and microRNA-218-5p (miR-218-5p) has been proven to play an important role in cancer development. However, the specific mechanism of miR-218-5p’s effect on the development of lung adenocarcinoma (LUAD) has not been clarified. Methods The expression of miR-218-5p in LUAD tissues was analyzed in TCGA data, and the mRNA expression of miR-218-5p and EGLN3 in human lung normal and human LUAD cell lines was detected by qRT-PCR. Western blot was used to detect the protein expression levels of key AKT/ mTOR signaling pathway proteins and EGLN3 in tumor cells. The microRNA-target relationship between miR-218-5p and EGLN3 was predicted by bioinformatics method and confirmed by luciferase reporter gene detection. The roles of miR-218-5p and EGLN3 in LUAD were measured by CCK-8, colony formation, scratch healing, and Transwell assays. Results miR-218-5p was underexpressed in LUAD. Overexpression of miR-218-5p inhibited the proliferation, migration and invasion of LUAD cells and the activation of the AKT/ mTOR signaling pathway. EGLN3 was the downstream target gene of miR-218-5p, which was highly expressed in LUAD. Overexpression of EGLN3 weakened the inhibitory effect of miR-218-5p on proliferation, migration and invasion by LUAD cells. Conclusions miR-218-5p inhibited proliferation, migration, and invasion in LUAD cells by negatively affecting EGLN3 expression.
HOU Yujiao , ZHANG Jie , ZHAO Chaoyang , XU Tianjiao , LI Meiqian , HONG Bo , LI Wenjing
2022, 32(11):68-74,112. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 009
Abstract: Objective The purpose of this study is to comprehensively elucidate the anti-hepatic fibrosis mechanism of ( Glycyrrhiza Uralensis and Salvia Miltrorrhiza,GUSM) from the perspective of endogenous metabolites. Methods Hepatic fibrosis rat model was established by subcutaneously injecting CCl4 solution. The efficacy of GUSM was evaluated by liver morphological indexes and HE method . We analyzed, screened and identified the metabolic profiles of rats from Normal group, Model group, GUSM group and Positive group by PCA and OPLS-DA method using UPLC-TOFMS. Results A total of 16 potential biomarkers in both positive and negative mode associated with hepatic fibrosis were screened and identified. The metabonomics analysis revealed that Taurocholic acidf Glycocholic acid, Arachidonic acid,and so on were greatly disturbed. Conclusions GUSM prescription showed anti fibrosis effect on rats through regulating metabolic pathways, mainly about Arachidonic acid metabolism. This study is useful to better understand and analyze the anti hepatic fibrosis mechanism of GUSM prescription on HF rats using metabonomics.
ZHANG Rui , ZHANG Jingdan , SHU Yao , XING Xiaowen , WANG Cui , LIU Zhiqiang , ZHANG Jianning
2022, 32(11):75-85. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 010
Abstract: Objective To explore the effects of CD44 gene knockout on behavioral abilities in mice by animal behavior experiment. Methods Beam balance experiment, rotarod experiment, open field experiment, elevated plus-maze experiment, tail suspension experiment, forced swimming experiment, novel object recognition experiment, three-chamber social experiment and Morris water maze experiment were carried out to analyze and compare the behavioral differences between homozygous C57BL/ 6J CD44 knockout (CD44 KO) mice and wild type (WT) mice. Results In Stage 3 of the three-chamber social experiment, WT female mice were seen to spend more time exploring novel objects than female mice in the CD44 KO group (P= 0. 0475). From day 5 to day 7 in the water maze navigation experiment, mice in CD44 KO group were observed to take longer to reach the target area than WT mice (P = 0. 0147, 0. 0182, 0. 0233). In the water maze space exploration experiment, mice in the CD44 KO group tended to reach the target area fewer times (P= 0. 0128) and took longer time to reach the target area for the first time (P= 0. 0003). The time that males in the CD44 KO group took to reach the target area for the first time was less than that taken by females (P= 0. 049). Additionally, males in the CD44 KO group took more time than WT males (P= 0. 0137), and females in the CD44 KO group also took more time than WT females (P= 0. 0036). There were no significant differences in the result of the beam balance experiment, rotarod experiment, open field experiment, elevated plus maze experiment, tail suspension experiment, forced swimming experiment and novel object recognition experiment between the two groups. Conclusions Compared with WT mice,CD44 KO mice were observed to have significantly decreased learning and memory ability, especially in the 5th~ 7th day.In addition, their spatial memory ability was obviously weakened. Meanwhile, CD44 KO female mice were seen to have weaker social propensity than WT female mice. However, no significant differences were noted between CD44 KO mice and WT mice in the aspects of sports coordination ability, balance ability, endurance, anxiety and depression.
YANG Ze , WANG Kezhou , YU Yang
2022, 32(11):86-94. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 011
Abstract:Sepsis is a common complication of severe injuries, such as severe infection, trauma, burn, and shock, and it is the main cause of death of critically ill patients. Endotoxemia caused by lipopolysaccharides (LPS), cell wall components of gram-negative bacteria, is one of the main causes of sepsis. Multiple plasma proteins, including lipopolysaccharide binding proteins ( LBP ) and bactericidal permeability increase proteins ( BPI ), are involved in regulating the signaling pathways of LPS activation. The two proteins belong to the same family of proteins with similar structures but different biological functions: LBP facilitates LPS in binding to the CD14 receptor of target cells to increase host sensitivity to LPS, while BPI neutralizes the inflammatory effects of LPS and accelerates the clearance of LPS from the circulation. In this review, we summarized the research progress of LBP and BPI in terms of structure, function, potential for treatment of sepsis, and the correlation between gene polymorphisms and sepsis.
HOU Yujun , WANG Kai , CHEN Ying , WANG Lu , JIANG Huiling , LI Ying , ZHOU Siyuan
2022, 32(11):95-100. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 012
Abstract:Intestinal barrier dysfunction is an important etiological and pathological feature of many diseases. The role of microRNA in intestinal barrier injury is the subject of more and more attention and increasingly focused research. Studies have found that microRNA can be involved in the destruction or protection of intestinal barrier function by combining different targets to regulate structural proteins, apoptosis, immunity, inflammation, and oxidative stress. The research is summarized, and different mechanisms of microRNA regulation of the intestinal barrier are discussed, to explore the potential value of microRNA when studying the mechanisms, diagnosis, and treatment of these diseases.
ZHAO Sha , LEI Xuan , XIONG Jiamin , YANG Shulong , HONG Fenfang
2022, 32(11):101-106. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 013
Abstract:Obstructive sleep apnea syndrome ( OSAS ) is a chronic disease of sleep-disordered breathing characterized by repeated collapse of part or all of the upper respiratory tract during sleep. Its clinical manifestations include fragmentation of sleeping, severe snoring, and excessive daytime sleepiness. Because of its high incidence and ability to affect the quality of life and survival rate of patients, increasingly more people have been attaching clinical importance to OSAS. Many recent studies have shown that OSAS is characterized by inflammation, and changes in the levels of inflammatory-related factors suggest the possibility that OSAS is complicated by cardiovascular disease. The potential relationship between OSAS and inflammation is demonstrated by the presence of common risk factors between the two conditions as well as several common pathogenicity approaches. Therefore, this study was performed to summarize the research progress on the correlation between OSAS and inflammation and thus increase our understanding of the pathogenesis of OSAS.
2022, 32(11):107-112. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 014
Abstract:The 3R principles of laboratory animal research have been universally recognized and applied worldwide for decades, but they are inherently flawed and cannot systematically protect the welfare of laboratory animals, save social costs, or create social benefits. In 2020, new principles of animal ethics research were proposed that inherited the 3R principles, with the composition of three principles of “social benefits” and “animal welfare” This paper analyzes the new principles of animal ethics and proposes a direction for future laboratory animal legislation in China from the perspective of the six new principles.
2022, 32(11):113-120. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 015
Abstract:Acute lung injury ( ALI) is one of the many life-threatening complications of sepsis, but its pathogenesis is still unclear. Establishing a stable and reliable preclinical model of sepsis ALI is a productive way to clarify its pathogenesis, explore potential therapeutic targets, and detect the safety and therapeutic effects of newly developed drugs. At present, the animal model of sepsis ALI in vivo is relatively mature. With improvements in scientific research technology, researchers around the world have built a variety of different cell models and lung organ chip models for studying the disease. In this paper, the research progress of three preclinical models of sepsis-related ALI, including animal, cell and lung-on-a-chip models, will be reviewed to provide a reference for those optimizing and selecting experimental models.
PENG Mengfan , LI Ming , MIAO Jinxin , TIAN Shuo , MIAO Mingsan
2022, 32(11):121-127. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 016
Abstract:Esophageal cancer is a common gastrointestinal cancer with high incidence and mortality rates. Because the early symptoms are not typical, most patients are in the middle and late stages when diagnosed, and the treatment effect is disappointing. At present, our understanding of the pathogenesis of esophageal cancer is limited; it is considered to be the result of genetic-environmental interactions, but there are no definite conclusion , and there is a lack of effective treatments. Therefore, clarifying the potential pathogenesis of esophageal cancer is of great significance for research into early clinical screening, diagnosis, treatment, and prognosis. Long noncoding ( LncRNA) is a new biomarker for tumor diagnosis, treatment, and prognosis evaluation. LncRNA plays an important role in tumor cell differentiation, proliferation, apoptosis, invasion, and metastasis and mediates the occurrence and development of a variety of malignant tumors, including esophageal cancer. LncRNA plays a dual role by inhibiting and promoting cancer in esophageal cancer, but the amount of relevant literature is limited, and the mechanism of LncRNA mediation of the occurrence and development of esophageal cancer is not completely clear. This paper systematically reviews the dual-regulation mechanism of LncRNA to clarify how it affects the occurrence and development of esophageal cancer and to provide new research ideas for the clinical prevention and treatment of esophageal cancer.
2022, 32(11):128-134. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 017
Abstract:Venous malformations (VM) are the most common type of congenital vascular malformations. At present, their pathogenesis has not been fully elucidated, and treatment strategies are not yet mature. Animal models play important roles in the study of the pathological mechanisms and treatment of VM. This paper summarizes the basic pathogenesis of VM and the research status of VM animal models at home and abroad, expounds several common model construction method and result , and discusses the advantages and disadvantages of the models to provide a reference for researchers when selecting and establishing experimental animal models.
WANG Xing , ZHAO Jingyi , ZHANG Yu , SHEN Luyan
2022, 32(11):135-141. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 018
Abstract:The ethics and 3R principle of experimental animals are basic tenets that should be strictly obeyed. However, because of the inner complexity of organisms, the data obtained in vivo tend to be gathered in voluminous amounts. There is a lack of appropriate method to thoroughly explore these datasets, which are too complicated and contain too much hidden information to be analyzed effectively. Therefore, the number of experimental animals performed is increasing. However, in the context of big data, and with the rapid development of computer technology, artificial intelligence (AI) has made splendid progress. Nowadays, AI is widely applied in the establishment of relevant databases and mathematical models and the identification and prediction of features of, e. g. , microscopic molecules, macroscale images, behavior, and basic physiological indicators, and it serves as a powerful and convenient auxiliary tool for scientific researchers. Compared with micro-analysis, the macroscopic application of character recognition seems more feasible and practicable. This paper summarizes the applications, challenges, and prospects of AI use in the recognition of phenomena hidden in experimental animal data.
ZHANG Yaqing , QI Feifei , BAO Linlin
2022, 32(11):142-148. DOI: 10. 3969 / j.issn.1671-7856. 2022. 11. 019
Abstract:Five of seven coronaviruses that cause respiratory disease in humans were beta-coronaviruses. HCoVOC43 and HCoV-HKU1 show seasonal transmission and cause mild symptoms after infection. SARS-CoV, MERS-CoV and SARS-CoV-2 spread rapidly from person to person, which posed a serious threat to public health security and national economy. Nucleic acid testing is the key test to identify, diagnose and screen people infected by virus, but the positive of viral loads does not mean the virus is contagious. It is urgenly to explore a rapid detecting method for virus replication as a supplement to judge virus activity. Studies have confirmed that the subgenomes produced by beta-coronaviruses during cell replication can be used as indicators for judging viral activity. This paper reviews the detection and application of subgenomes of beta-coronavirus, aiming to establish subgenome detection method , optimize virus detection method , perfect experimental animal model creation and drug or vaccine effectiveness evaluation systems and help to carry out experimental research.