Abstract:Objective Shkbp also called Shkbp1, can competitively inhibit binding CIN85 and c-Cbl, thereby blocking the epidermal growth factor receptor (EGFR) endocytosis and degradation, to play its role of tumor promotion. This article aims to explore Shkbp1 deletion (Shkbp-1 - / -) mice Changes in blood, bone marrow, spleen and blood cell classification T cell subsets. Methods Shkbp-1 - / - transgenic mice were identified by PCR genotype; blood tests using automatic classification of blood cells was measured using flow cytometry Shkbp-1 - / - and control mice blood, bone marrow, and spleen T lymphocyte subsets. Results Blood routine examinations showed that: neutrophils and eosinophils tended to increase, and there are significant differences. Streaming results showed that: the control group reduced blood CD4 CD8 double positive cells in the bone marrow CD3 , CD4 increased. In the spleen tissues show its CD3 , CD4 , CD8 , CD4 CD8 double positive cells were decreased. Conclusion Shkbp1 involved in the maturation and differentiation of blood cells, which affects the number of immune cells. This study lays a foundation for the study of how Shkbp1 is involved in the differentiation of blood cells.

Abstract:Objective Shkbp is also called Shkbp1, can competitively inhibit binding CIN85 and c-Cbl, thereby blocking the epidermal growth factor receptor (EGFR) endocytosis and degradation, to play a role in tumor promotion. This study aims to explore the changes in blood cell classification and T cell subsets in blood, bone marrow, and spleen in Shkbp1-deletion (Shkbp-1-/-) mice. Methods Shkbp-1-/- transgenic mice were identified by PCR genotyping. Blood cell classification was performed using an automatic classification system. Flow cytometry was used to detect the T lymphocyte subsets in the blood, bone marrow, and spleen of Shkbp-1-/- and control mice. Results Routine blood examination showed that neutrophils and eosinophils tended to increase and showing significant differences, and there was no significant difference in lymphocytes. The flow cytometry results showed that there was a decrease of CD4+CD8+double positive cells and increase of bone marrow CD3+ and CD4+ cells in the control group. However, there was a decreasing trend of CD3+, CD4+, CD8+, and CD4+CD8+cells in the spleen tissues. Conclusions Shkbp1 is involved in the maturation and differentiation of blood cells, and affects the number of immune cells. This study lays a foundation for the study of how Shkbp1 is involved in the differentiation of blood cells.