Abstract: Objective A neuroblastoma (NB) liver transplantation model was established and compared with the adrenal orthotopic transplantation model to explore its characteristics. Methods 5 × 105 SK-N-SH cells were implanted along the long axis of the left lobe of mouse livers with a micro-injection needle. The growth, metastasis, expression of related genes, and histopathological changes of tumors were detected after the modeling. Results The tumor formation rate in mice inoculated with tumor cells reached 100% after 21 days, and tumor growth, metastasis, related gene expression changes, and pathological characteristics were apparent. Conclusions In this study, a neuroblastoma liver transplantation model was successfully constructed via a relatively simple surgical method to provide a more suitable choice for future scientific NB experiments.