A pair of primers were designed based on published sequence of BAI\|A strain of AMV and the total RNA of virus was extracted from different tissues of specific\|pathogen\|free(SPF)chickens which had been challenged with avian myeloblastosis virus.The P27 gene segment of group\|specific antigen of AMV was amplified by RT\|PCR.The result showed that the RT-PCR method is high specific and sensitive in detection of AMV from feather pulp.This study discussed the detection of AMV with PCR and the distribution of AMV in different tissues preliminarily.