Objective To determine the effect of DNCB in the Nc/Nga mice and to explore the ways of establishing AD models.Methods DNCB was repeatedly applied to the ear of NC /Nga mice.Atopic dermatitis-like skin lesions were evaluated by ear thickness,skin histology and immunological parameters.Results Repeated application of DNCB caused rapid increase in ear thickness.Clinical and histological symptoms(dermal and epidermal thickening,hyperkeratosis,parakeratosis and inflammatory cell infiltration) in this model were...