Objective To improve the method of establishing model in myocardial ischemic reperfusion rat.Methods 32 SD male rats were divided into four groups randomly(Group A:sham group,Group B:myocardial ischemic,Group C:ischemic 30 min and reperfusion,Group D:ischemic 60 min and reperfusion).Inspected the changing of ECG in preparation /in-operation and rats breathing were finished under the control of artificial respirator in the period of MI.After 72 h of the operation,the levels of myocardial zymogram were determ...