ObjectiveTo establish a loop-mediated isothermal amplification (LAMP) technique for detecting Staphylococcus aureus in laboratory animals. MethodsFour primers were designed and screened specifically to recognize the nuc gene of Staphylococcus aureus. Optimal reaction conditions were screened. Results2 cfu extracted crude DNA and no cross reaction with other commonly seen pathogenic bacteria was found when the conditions were 60℃ for 40 min at least, Mg²⁺at a concentration of 8.0 mmol/L, dNTP 2.0 mmol/L and betaine 0.8mmol/L. The result of reaction can be directly observed with naked eye by adding a fluorescent SYBR green I dye. ConclusionsThis LAMP technique for detectiong Staphylococcus aureus is sensitive, rapid and simple to perform, and can satisfy the requirements of grassroots lab, mass samples and quick detection.