The Design and Making of an Experimental Apparatus for Rat Climbing Board Test and Application in the Rats with Cerebral Injury
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    Abstract:

    ObjectiveTo design and make an experimental apparatus so that the researchers can easily and quickly detect the neural function deficits of rats after cerebral injury by means of climbing board test, and to improve the precision of the experimental data. MethodsThe male adult SD rats were randomly divided into three groups:Tβ4 group, PBS group and Sham group. From the 1st to the 7th day after operation, the new apparatus and the primary one were applied separately in the climbing board test to detect the neural function deficits of rats of the three groups. The nerve function loss score was recorded and analyzed by statistical methods. ResultsResults based on the new apparatus:Both the nerve function loss score of the Tβ4 group and that of the PBS group decreased gradually, and the neural function deficits could still be detected five days later. The statistical difference (P<0.05)between the two groups could be checked out three days later. The statistical difference (P<0.05)between the Tβ4 group and the Sham group could be detected in the earlier three days. The statistical difference (P <0.05)between the PBS group and the Sham group could be found during the seven days after operation. Results based on the primary apparatus:Both the nerve function loss score of the Tβ4 group and that of the PBS group decreased sharply, and the neural function deficits couldn''t be detected five days later. The statistical difference between the two groups could be found only at the 4th day. The statistical difference (P<0.05)between the Tβ4 group and the Sham group could be detected only in the earlier two days. The statistical difference (P<0.05)between the PBS group and the Sham group could be found only in the earlier four days. ConclusionThe new experimental apparatus is easily to be made and can be operated conveniently in experiment. It can increase the precision of experimental data, and has better function to detect the statistical differences resulted from the experimental factors.

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