Establish and Verification of Transgenic EGFP Nude Mouse
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    Abstract:

    ObjectiveTo develop a transgenic GFP nude mouse model, human lung carcinoma cells were implanted to test and verify the Immunodeficiency of the model, then observing the result of Dual-color fluorescence imaging. MethodThe GFP nude mouse was obtained by crossing BALB/c male nude mice with the transgenic EGFP female C57/B6 mouse. We observe the Thymus through Gross Anatomy, test GFP expression Through Whole-Body and Major Organs Fluorescence Imaging.Six-week-old nude EGFP mouse was injected RFP-expressing Human A549 lung carcinoma cells (RFP-A549) subcutaneously with a single dose of 2×106, test and verify the Immunodeficiency of EGFP-expressing nude mice through The development of tumor. Meanwhile, a GFP mouse-RFP tumor model was established to evaluate the Dual-color fluorescence imaging. ResultDeveloped EGFP-expressing nude mice, obvious fluorescence can be observed in whole-body and major organs include brain, heart, lungs, liver, kidney, intestines, stomach and pancreas. After implanted RFP-A549 cells subcutaneously, the percentage of tumorigenesis is 90%, fluorescence imaging show dual-color clearly. ConclusionDeveloped a transgenic GFP nude mouse model, the whole-body fluorescence imaging clearly and have Immunodeficiency.

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