Objective To investigate the influence of different week-age and the hormonal level on superovulation in Mongolian gerbils. Methods Eight groups of Mongolian gerbils at different age of 4-18 weeks were induced to superovulate by intraperitoneal injection of 10 IU PMSG/HCG, respectively. At 16-17 h after the last injection, the amounts of eggs of animals in different groups were counted and analyzed to determine the best week-age of superovulation. Superovulation was induced in the animals of best week-age by intraperitoneal injection of 5,0,15 IU PMSG/HCG to analyze the different amounts of eggs. ResultsCompared with other groups, the amount of eggs obtained in gerbils at age of 6 weeks is highest and there were significant differences among all groups (P<0.05). The amounts of eggs obtained in groups of different PMSG/HCG doses were significantly different and that of the 10 IU group was highest. ConclusionThe age of 6 weeks and the dose of 10 IU PMSG/HCG are the best ages and effective dose to induce superovulation in Mongolian gerbils.