Objective To explore the relationship between the gait behavior changes and cognitive function in APP/PS1 transgenic mice. Methods 16 APP/PS1 transgenic mice were divided into model group and Huperzine A group, C57/BL6J mice with the same age were chosed as control group. After a 150 days consecutive treatment, Morris water maze(MWM) was used to detect the learning and memory ability and Gait analysis system (GAS-2) was used to detect the gait behavior after the treatment when the mice were 8-month-old. Results The escape latency of the model group was significantly higher than that of the control group (P< 0.05), the time spended in the target quadrant, swimming distance in the target quadrant significantly lower than that of the control group (P< 0.05), the first time passing through the platform prolonged significantly than the control group (P< 0.05), and the number of passing though the platform reduced significantly than the control group (P< 0.05). In the gait behavior experiments, compared with the control group, the average walking speed of the model group reduced significantly (P< 0.05), the average walking cycle, the absolute average body angle and lateral movement increased significantly (P< 0.05); The percentage of support time in a walking cycle of the left and right foot increased significantly (P< 0.05). Accordingly, the percentage of swing time in a walking cycle of the left and right foot reduced significantly (P< 0.05). The propulsion index of the left hand, right hand, right foot increased significantly (P< 0.05), and then the braking index of the above three feet decreased significantly (P< 0.05). Huperzine A can improve the cognitive function, rectify the changes in the gait behavior. The two behavioral relevance shows that cognitive function and the front two feet braking, propulsion index have a high correlation index (correlation coefficients were -0.433, -0.379, P values were 0.039,0.079), the others were not. Conclusion APP/PS1 transgenic mice of 8-months-old have a remarkable impairment of learning and memory ability and disorder of gait behavior, and these two behaviors have a correlation in some extent.