Objective To measure the organ weights, physiological and biochemical parameters and immune cells of NOD/SCID mice. Methods Mice at five and ten weeks of age were selected. The organ weights, blood physiological and biochemical parameters were observed. The percentages of CD3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were checked by FCM in NOD/SCID mice at six week of age in terms of its T, B lymphocyte function and NK cell activity. Results Among the same sex group of NOD/SCID mice, the weights of kidney, liver, heart and lung, and the blood physiological indexes of RBC, HGB, HCT, MCV, MCH, RDW, and the blood biochemical indexes of TP, ALB, ALP, CHO, TBIL show significant difference between 5 and 10 weeks. At the same age, HCT, GLOB, A/G, CHO, TG, TBIL and the UN are significantly different between male and female NOD/SCID mice. NOD/SCID mice lack T cells (0.37±0.26)%、CD4+T cells (0.35±0.13)%、CD8+T cells (0.47±0.10)%、CD19+B cells (0.13±0.05)%、B220+B cells (1.20±0.44)%. The percentage of NK cells is (6.90±0.82)%, and the percentage of granulocytes is (47.88±15.54)%. Conclusions The study indicates that NOD/SCID mice show the deficiency of T, B and NK cells function. The organ weights, blood physiological and biochemical parameters are affected by age and gender. The studied parameters of NOD/SCID mice are similar with the same strain in other countries.