Objective To research the method of Chronic hepatic injury modeling in mice induced by D-galactosamine and lipopolysaccharide combination. Methods Injected D-galactosamine (30 mg/mL) and lipopolysaccharide (2μg/ mL) combination by intraperitoneal injection, two days at a time for 8 weeks. Monitored variation of diet and weight; detected serum level of alanine aminotransferase(ALT) and aspartate aminotransferase(AST), been put to death in mice and removed the liver tissue. strained hepatic tissue by the HE and Masoon dye to observe Liver tissue structure and cellular morphology and the degree of fibrosis.Results Lipopolysaccharide and D-galactosamine combination resulted in ALT rise, hepatocyte degeneration and necrosis,collagen fiber hyperplasia obviously.Conclusion D-galactosamine and Lipopolysaccharide combination could induce mice chronic hepatic injury modeling.