Objective To improve the accuracy of detection through analyzing the phenotypes of P. pneumotropica isolates in laboratory animals in Beijing area. Methods 306 suspicious P. pneumotropica strains were identified by biochemical identification and 16S rDNA sequencing. Then, to obtain the phylogenetic relationships combined with colony characteristics on blood agar plates and biochemical characteristics of 53 biotypes. Results BD Phoenix 100 automated bacterial identification system and 16S rDNA sequencing identified P. pneumotropica positive rate of 306 isolates were 164/306 and 227/306, respectively. There were 140 phenotypes in 227 true-positive strains, of which 106 were biotype Heyl and 23 were biotype Jawetz.Conclusions In the samples of laboratory animals in Beijing area, P. pneumotropica infection mainly are of biotype Heyl, and less is of biotype Jawetz. The phenotypes are diverse and widely distributed.