In vivo imaging of <sup>99m</sup>Tc-Glucarate-labeled acute necrotic myocardium using microSPECT/CT

doi:10.3969.j.issn.1671.7856. 2014.007.003

Home > Archive>Volume 24, Issue 7, 2014 >14-18. DOI:10.3969.j.issn.1671.7856. 2014.007.003

In vivo imaging of 99mTc-Glucarate-labeled acute necrotic myocardium using microSPECT/CT
DOI:
                        10.3969.j.issn.1671.7856. 2014.007.003
                    
CSTR:
                        
                    
Author:
                        BAO Lu-erBAO Lu-er
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHANG LinZHANG Lin
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHOU Xiao-huiZHOU Xiao-hui
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
MENG Qing-shuMENG Qing-shu
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHAN Zhen-zhenZHAN Zhen-zhen
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
FAN Hui-minFAN Hui-min
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:
Clc Number:
Fund Project:

Article

Figures

Metrics

Reference [17]

Cited by

Materials

Comments

Abstract:

Objective To evaluate the anatomic localization and size of acute necrotic myocardium in the ischemic-reperfused rat hearts using ^99mTC-Glucarate and microSPECT/CT. Methods The ischemic-reperfused (IR) rat heart models were established by ligating left anterior descending coronary artery for 60 min. ^99mTC-Glucarate was intravenously injected into the rats 24 hours after IR operations. Images were acquired 30 min after administration of ^99mTC-Glucarate using microSPECT/CT. Anatomic localization and size of acute necrotic myocardium were analyzed with microSPECT/CT imaging,and these results were compared to those determined by triphenyltetrazolium chloride (TTC) staining. Results The microSPECT/CT images showed hot spot accumulations of ^99mTC-Glucarate in IR hearts (the heart-to-liver ratio was 1.90±0.33), not in controls (P < 0.05). The anatomic localization of ^99mTC-Glucarate-labeled necrotic myocardium were in correspondence with TTC staining results. The hot spot size was related significantly to necrotic myocardial size determined by TTC staining (R²=0.964). Conclusions The localization and size of acute necrotic myocardium can be assessed by non-invasive microSPECT/CT imaging with^99mTc-Glucarate.

Key words:Rats;Ischemic-reperfusion injury;^99mTc;Glucarate;microSPECT/CT

Reference

[1] Orlandi C, Crane PD, Edwards DS, et al. Early scintigraphic detection of experimental myocardial infarction in dogs with technetium-99m-glucaric acid[J]. Journal of nuclear medicine: official publication, Society of Nuclear Medicine 1991, 32(2):263-268.

[2] Khaw BA, Nakazawa A, O'Donnell SM, et al. Avidity of technetium 99m glucarate for the necrotic myocardium: in vivo and in vitro assessment[J]. Journal of nuclear cardiology: official publication of the American Society of Nuclear Cardiology 1997, 4(4):283-290.

[3] Narula J, Petrov A, Pak KY, et al. Very early noninvasive detection of acute experimental nonreperfused myocardial infarction with 99mTc-labeled glucarate[J]. Circulation 1997, 95(6):1577-1584.

[4] Arteaga de Murphy C, Ferro-Flores G, Villanueva-Sanchez O, et al. 99mTc-glucarate for detection of isoproterenol-induced myocardial infarction in rats[J]. International journal of pharmaceutics 2002, 233(1-2):29-34.

[5] Mariani G, Villa G, Rossettin PF, et al. Detection of acute myocardial infarction by 99mTc-labeled D-glucaric acid imaging in patients with acute chest pain[J]. Journal of nuclear medicine: official publication, Society of Nuclear Medicine 1999, 40(11):1832-1839.

[6] Wyffels L, Gray BD, Barber C, et al. Detection of myocardial ischemia-reperfusion injury using a fluorescent near-infrared zinc(II)-dipicolyla mine probe and 99mTc glucarate[J]. Molecular imaging 2012, 11(3):187-196.

[7] Okada DR, Liu Z, Johnson G, et al. 99mTc-glucarate kinetics differentiate normal, stunned, hibernating, and nonviable myocardium in a perfused rat heart model[J]. European journal of nuclear medicine and molecular imaging 2010, 37(10):1909-1917.

[8] Liu Z, Barrett HH, Stevenson GD, et al. High-resolution imaging with (99m)Tc-glucarate for assessing myocardial injury in rat heart models exposed to different durations of ischemia with reperfusion[J]. Journal of nuclear medicine: official publication, Society of Nuclear Medicine 2004, 45(7):1251-1259.

[9] Ohtani H, Callahan RJ, Khaw BA, et al. Comparison of technetium-99m-glucarate and thallium-201 for the identification of acute myocardial infarction in rats[J]. Journal of nuclear medicine: official publication, Society of Nuclear Medicine 1992, 33(11):1988-1993.

[10] Khaw BA. The current role of infarct avid imaging[J]. Se minars in nuclear medicine 1999, 29(3):259-270.

[11] Johnson G, 3rd, Okada CC, Hocherman SD, et al. (99m)Tc-glucarate imaging for the early detection of infarct in partially reperfused canine myocardium[J]. European journal of nuclear medicine and molecular imaging 2006, 33(3):319-328.

[12] Strauss HW. Myocardial perfusion imaging: perspectives from a turbulent twenty-five years[J]. The quarterly journal of nuclear medicine: official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR) 1996, 40(1):20-26.

[13] Gerson MC, McGoron AJ. Technetium 99m glucarate: what will be its clinical role?[J]. Journal of nuclear cardiology: official publication of the American Society of Nuclear Cardiology 1997, 4(4):336-340.

[14] Perek N, Sabido O, Le Jeune N, et al. Could 99mTc-glucarate be used to evaluate tumour necrosis? In vitro and in vivo studies in leukaemic tumour cell line U937[J]. European journal of nuclear medicine and molecular imaging 2008, 35(7):1290-1298.

[15] Gambini JP, Cabral P, Alonso O, et al. Evaluation of 99mTc-glucarate as a breast cancer imaging agent in a xenograft animal model[J]. Nuclear medicine and biology 2011, 38(2):255-260.

[16] Gambini JP, Nunez M, Cabral P, et al. Evaluation of patients with head and neck cancer by means of 99mTc-glucarate[J]. Journal of nuclear medicine technology 2009, 37(4):229-232.

[17] Choudhury PS, Savio E, Solanki K, et al. 99mTc Glucarate as a Potential Radiopharmaceutical Agent for Assessment of Tumor Viability: From Bench to the Bed Side[J]. World journal of nuclear medicine 2012, 11(2):47-56.

Get Citation

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:May 20,2014
Adopted:
Online: August 19,2014
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Related Videos

Share

Article Metrics

History

Article QR Code