LIU Su-su
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaWU Xi
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaZHOU Shu-ya
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaWANG Chen-fei
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaPENG Ze-xu
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaZUO Qin
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaLI Bao-wen
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaHE Zheng-ming
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaFAN Chang-fa
National Rodent Laboratory Animal Center, National Institute of Food and Drug Control, Beijing 100050, ChinaObjective To obtain the basic growth parameters of a self-established F1 hybrid CB6F1 C57-ras transgenic mouse model, and to provide basic information for commercialization of this mouse model. Methods F1 hybrid mice (CB6F1) were produced by crossing C57-ras heterozygous transgenic (c-Ha-ras+/-) male mice and wild-type BALB/cJ female mice. The average litter size, weaning rate, sex ratio, growth performance and C57-ras transgenic positive rate were recorded and analyzed. Results The average litter size was eight, weaning rate was 90%, and sex ratio was approximately in accordance with prediction. The average body weight of newborn mice was 1.73±0.05 g. The average body weight of 10-week old c-Ha-ras transgenic female and male mice in CB6F1 background was 24.38±1.74 g and 29.42±1.72g, respectively, which had a significant difference (P<0.01). The c-Ha-ras transgenic positive rate was 46.9%. which was in accordance with genetic rules. Conclusions The F1 hybrid mice (CB6F1) produced by crossing C57-ras heterozygous transgenic (c-Ha-ras +/-) male mice and wild-type BALB/cJ female mice show normal growth performance and development characteristics, and it can be used for large-scale commercial supply.
