Objective To compare the differences of two stocks of guinea pigs, the albino guinea pigs and pigment guinea pigs, in establishing dyslipidemic model, to evaluate their lipid-lowering action, and to compare their properties for development of hyperlipidemia. Methods Two stocks of the 5-week-old guinea pigs were randomly divided into two groups, normal group (NC) and model group (Model). For the NC group, 12 guinea pigs were fed with normal chew. For the model group, after fed with high-fat diet for four weeks, 24 male guinea pigs were randomly grouped and treated with vehicle (VC group) and pitavastatin (Pit group) calcium, respectively, by gavage as well as received high-fat diet. Before and after modeling and pitavastatin treatment, blood samples were collected and subjected to analysis of plasma TC, TG, HDL-C and LDL-C, respectively. Results In the normal group, the blood lipid levels of albino guinea pigs were more stable than that of the pigmented pigs with the increase of age. After fed with high-fat diet, the plasma lipid levels of TC, TG and LDL-C were significantly increased in the two strains of guinea pigs, while HDL-C showed a decrease to varying degrees. Interestingly, the lipid level in the albino guinea pigs was significantly higher than that of pigment guinea pigs. And also, after drug administration for four weeks, pitavastatin treatment significantly decreased the elevated lipid level of TC, TG and LDL-C in the albino guinea pigs compared with that in the pigment guinea pigs. Conclusions The albino guinea pigs and pigment guinea pigs demonstrate certain differences in establishing dyslipidemic model and evaluating lipid-lowering pharmacodynamics. However, compared with the pigment guinea pigs, the albino guinea pigs have obvious superiority because of easy establishment of hyperlipidemia model and are more sensitive to lipid-lowering drugs.