Objective To explore the modified increasing intracranial pressure method to set up the brain death model for rabbits and sustain the state of brain death. Methods 15 healthy New Zealand rabbits were divided into brain death group (Group A,n=9) and sham operation group(Group B,n=6) randomly. The 9 brain death rabbits were set up by increasing intracranial pressure in a modified way. The sham operation rabbits were only sustained with anesthesia.Recorded the mean artery pressure (MAP) and heart rate (HR). Results In group A, the 8 brain death rabbits were successfully set up. Only 1 died of heart failure. Compared to Group B, the MAP and HR of Group A rise up greatly during increasing intracranial pressure and presented an increasing trend. The peak MAP and HR were (111.63±7.71) mmHg and (262.38±16.60)beats/min which were much higher than the MAP(80.63±8.45) mmHg and HR(153.25±14.35) beats/min before increasing intracranial pressure(P < 0.05). After brain death, MAP decreased sharply to 45mmHg. 2 hours after brain death, HR (218.50±10.27) beats/min presented an decreasing trend too, which showed a significantly statistic difference to peak HR(262.38±16.60) and HR(153.25±14.35) beats/min before brain death(P < 0.05). Conclusions Compared to traditional method, the brain death model could be established stably and securely in the modified way and maintained for long time with the respiration and circulation supports.