Objective To investigate the therapeutical effect of Ginkgo Biloba extract (GBE) on different ages of parkinsono Biloba e (PD) mice that induced by MPTP. Methods Sixty adult mice (8 weeks) and sixty older mice (32 weeks) were randomly divided into 6 groups: negative group, model group, positive group, high, medium and low dose GBE group. PD animal model were built through injecting with MPTP and then treated with different drugs accordingly. The limb motor function of mice were tested by using pole-climbing, hanging and swimming test. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of malondial dehyde (MDA) in the brain tissue were measure using spectro photography. The number of positive neurons expressing tyrosine hydroxylase (TH) in substantial nigra pars compacta and dopamine transporters (DAT) in striatum were detected by using the method of immunohistochemistry. Results 1. The motor function score, the activity of GSH-Px, and the mean optical density of TH and DAT in brain tissues of model group of 8 weeks significantly decreased compared with negative group, while the content of MDA was significantly raised, showing the model of PD was successfully established. The function score, the activities of SOD and GSH-Px, the mean optical density of TH and DAT in brain tissues of the GBE treatment group significantly raised compared with the model group, while the content of MDA was significantly decreased. 2. The motor function score, the activity of GSH-Px, and the mean optical density of TH and DAT in brain tissues of model group of 32 weeks significantly decreased compared with negative group, while the content of MDA was significantly raised, showing the model of PD was successfully established. The function score, the activities of SOD and GSH-Px, the mean optical density of TH and DAT in brain tissues of the GBE treatment group significantly raised compared with the model group, while the content of MDA was significantly decreased. Conclusions The preventive medication of GBE shows a protective effect on mice of different ages with PD induced by MPTP.