Objective To evaluate the feasibility and safety of transjugular liver biopsy(TJLB)by using the LABS 200 liver access and biopsy set (Cook Inc, USA).Methods Five minipigs were operated though TJLB puncture under the imaging guidance. The liver biopsies were analyzed by histological examination. Results Technical success of TJLB was achieved in all the 5 minipigs. No procedure-related complications occurred, and sufficient amount of specimen for histological examination was obtained in all cases.Conclusions Our preliminary results indicate that transjugular liver biopsy with the use of Cook LABS 200 liver access and biopsy set is clinically safe and feasible, and provide technical support for its clinical application.