According to relevant national laws and regulations, practitioner training was included into laboratory animal science teaching reform. By adjusting the training content and teaching method and use of animal models of typical human diseases, the transformation of training mode was realized and improved. By the assessment of basic theory in combination with practical operation, the thinking ability and hands-on skill of the practitioners are much improved. Through classroom instruction, experimental teaching, quality assessment and tracking survey, the evaluating process of the training quality of training teaching is performed. Therefore, the teaching reform of the laboratory animal science based on the training of practitioners is established.