Objective To investigate effects of post-treatment on stability of storage solution/thermostability, anti-calcification, the biomechanics and fibred structures of tannic acid fixed bovine jugular vein(BJV). Methods Fresh bovine jugular vein were treated with glutaraldehyde (Glut group), tannic acid (TA group), and post-treatment on TA-fixed (Study group). Calcium level was measured by implanting BJVs subcutaneously into the rats. The shrinkage temperature, tensile strength,elongation at break and histological changes were evaluated as well. Results Compared with Glut group,all results were better in Study group (P <0.05). Compared with TA group, in the Study group, color of storage solution did not change, shrinkage temperature and 60-days' calcium level decreased, 21-days' calcium level increased (P <0.01); biomechanical properties were not statistics difference between TA group and Study group(P > 0.05), and collagen fibers and elastic fibers were maintained well in TA group and Study group. Conclusion Post-treatment on TA-fixed Bovine Jugular Vein can improve stability of storage solution and BJVs elasticity, and do not change anti-calcification, biomechanical properties and fibred structures of TA-fixed BJVs.