Comparison of two types of myocardial infarction models in Beagle dogs
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    Abstract:

    Objective The purpose of this study was to compare two types of myocardial infarction (MI) models in Beagle dogs. Methods 30 dogs were divided randomly into three groups (n=10). ① The sham-operated group underwent pericardiotomy but without coronary artery ligation. The other two model groups were made under video-assisted thoracoscopy (VATS), where the left anterior descending coronary artery was closed by titanium clips: ② The direct vision group: the minimally invasive closure of the artery was performed under direct vision. In this group, the thoracoscopic operation was performed through a 3.0 cm small incision opened at the margin of the left third rib. ③ The thoracoscopic group: the video-assisted thoracoscope was inserted into the chest through a 1.0 cm exploratory hole in the midline of the third rib, and the surgical instruments were inserted through two 0.5 cm operating holes at the para-sternum line of the third rib and midclavicular line of the fourth rib. Electrocardiogram (ECG) was recorded and the levels of serum creatine kinase-MB (CK-MB) and troponin I (cTnI) were measured after modeling. The heart tissue samples were examined by histology using HE staining. The success rate of model establishment, durations from skin incision to chest closing and the wound healing were recorded. Results Compared with the sham-operated group, changes of myocardial infarction were observed in the two model groups (ECG S-T segment elevation, increased serum CK-MB and cTnI levels, myocardial ischemia and fibrosis, and reduced amount of cardiomyocytes). The survival rate was 90% in both of the two model groups. The operating time was shorter in the minimally invasive surgery under direct vision group, and the wound healing time was shorter in the thoracoscopic group. Conclusions The myocardial infarction models generated by minimally invasive surgery have less trauma and low mortality in the dogs. This model is suitable for investigation of pathophysiological mechanism associated with myocardial infarction.

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History
  • Received:
  • Revised:March 04,2016
  • Adopted:
  • Online: June 30,2016
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