The effect of vasoactive intestinal peptide (VIP) on the regulation of CD4+CD25+Treg/Th17 balance in the prevention and treatment of experimental autoimmune cerebrospinal meningitis (EAE)
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    Abstract:

    Objective To explore the effect of vasoactive intestinal peptide (VIP) on the regulation of CD4+CD25+Treg/Th17 balance in the prevention and treatment of experimental autoimmune cerebrospinal meningitis (EAE). Methods 60 healthy female Wistar rats were randomly divided into normal control group, EAE control group, VIP low-dose group and VIP high-dose group. Used of myelin basic protein (MBP)+completely adjuvant (CFA) to establish EAE model. Since building on, the low and high-dose VIP groups were with intraperitoneal injection of every other day respectively VIP 4 nmol/kg (0.2 mL), 16 nmol/kg (0.8 mL), normal control group and EAE group with 0.8 mL saline, 10 days in a row. Record the incubation period, progression and the peak of neurological dysfunction score (NDS) changes of rats; the pathological changes in brain at the morbidity peak of rats; the proportion of CD4+CD25+Treg/Th17 cells in spleen tissues with Flow cytometry; the cytokine levels of TGF-β1、IL-17A in brain tissue. Results the incubation period were extended, the progression and the peak nerve dysfunction score were shortened in VIP each dose group; In normal control group, there was no inflammatory cell infiltration in the brain tissue. The degree of infiltration of inflammatory cells in the VIP control groups were significantly lower than that in the EAE group;Compared with the EAE control group, the proportion of CD4+CD25+Treg cells in the spleen tissue of VIP each dose group increased, the proportion of Th17 cells decreased, and being a dose-response relationship;The cytokine levels of TGF-β1 in brain tissue were increased and the levels of IL-17A were reduced in VIP each dose group, being a dose-response relationship; Conclusion through up-regulating of CD4+CD25+Treg cells, promoting the secretion of TGF-β1 factor and cut ting the proportion of Th17 cells, inhibiting the expression of cytokines IL-17A, VIP reduced brain tissue inflammatory cell infiltration, and played a role in prevention and treatment of EAE.

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History
  • Received:
  • Revised:July 28,2016
  • Adopted:
  • Online: November 18,2016
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