In this study, a Specific Pathogen-Free (SPF) Bama miniature pig herd was established. The standards for breeding experimental pigs, genetic test and microbiological quality control had been drafted. The local standards of Heilongjiang province “SPF” technical specifications for microbiological monitoring of pigs had been formulated. The genetic and microbiological quality criterion had been used to control of the SPF pig herd. Classical swine fever virus (CSFV) and highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) infection models of Bama miniature pigs were established, especially the infection miniature pig model of PRRSV was used to evaluated commercially vaccine. The comparison of the cytokine homology between Bama miniature pigs and domestic pigs showed that the Bama miniature pigs can be used to instead of domestic pigs as an ideal experiental animal. At present the SPF Bama miniature pig colonies have been widely used to study the mechanism of prevention and pathogenic in the classical swine fever virus, porcine reproductive and respiratory syndrome virus and porcine transmissible gastroenteritis virus and porcine circovirus and so on. The completion of the project solved the bottleneck problem of the experimental pig usage which provided a new ideal experimental animal for animal disease and life science research.