Objective To explore whether CD4⁺T cells in mice can secrete exosomes and the possibility of exosomes to participate in the inflammation and immune response process. Methods Spleen samples were taken from mice, and CD4⁺T cells were isolated from the spleen tissue using magnetic bead separation method. Exosomes of CD4⁺ T cells were extracted from the supernatant and observed under transmission electron microscope. PCR assay was used to detect the expression of miR-23a and miR-214. Results Exosomes were observed under elecron microscope,and the expression of miR-23a and miR-214 were detected by PCR assay.Conclusions CD4⁺T cells in mice can secrete exosomes,which offer possibility to participate in the inflammation and immune response.