Objective To investigate and analyze the characteristics of blood physiological and biochemical parameters of Tibetan chickens bred in Guangzhou. Methods Blood samples of Tibetan chickens bred in Guangzhou were collected, and the physiological and biochemical parameters were measured. Results (1) The blood RBC, PLT, PDW, RDW-SD and P-LCR were not significantly different in the males than females ( P > 0.05). (2) HCT ( P < 0.05), MCHC ( P < 0.05), MPV ( P < 0.05), HGB ( P < 0.01), MCV ( P < 0.01) and MCH ( P < 0.01) were significantly higher between the males and females. (3) RDW-CV was significantly lower in the blood physiological parameters of males than females. (4) AST, TRIG, ALKP, ALT, Ca, CHOL, CREA, GLU, PHOS and TBIL were not remarkably different in the blood of males than females ( P > 0.05). (5) The blood AMYL ( P < 0.05) and TP ( P < 0.01) were significantly higher in the males than females. (6) The blood ALB ( P < 0.01), UREA ( P < 0.05), and GLOB ( P <0.01) were significantly lower in the males than females. Conclusions The essential data of blood physiological and biochemical indexes of Tibetan chickens bred in Guangzhou are obtained.