Establishment and application of a PCR detection method for Tupaia (tree shrew) paramyxovirus (TPMV)
Author:
Affiliation:

(Institute for Laboratory Animal Resources, National Institute for Food and Drug Control; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China)

Clc Number:

R-33

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To establish and preliminarily apply an effective PCR assay for detection of Tupaia (tree shrew) paramyxovirus (TPMV). Methods Using TPMV genomic DNA from NCBI GenBank, bases 8231 to 8720 were synthesized and inserted into a plasmid as a positive standard. One primer pair was designed based on this sequence. In total, 60 respiratory swabs and 12 lung tissues from the tree shrews were tested in this PCR assay. Results A PCR method for detection of TPMV was successfully established, with high specificity and sensitivity of 11.5 × 10 -5 μg/ mL. PCR result testing 60 respiratory swabs and 12 lung tissues were negative. Conclusions PCR for detecting TPMV has good specificity and high sensitivity and can be used for conventional tree shrew paramyxovirus detection.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:October 31,2017
  • Revised:
  • Adopted:
  • Online: June 26,2018
  • Published: