Revised fixation method for common carotid artery intubation in New Zealand rabbits
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(1. The Second Affiliated Hospital of Bengbu medical college,Bengbu 233000,China; 2. 123th Hospital of PLA, Bengbu 233015; 3. University of science and technology of China, Hefei 230026; 4. Department of Cardiopulmonary Rehabilitation Center, 128th Hangzhou Hospital, Hangzhou 310007; 5. Hangzhou sanatorium,Hangzhou 310007)

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R-33

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    Abstract:

    Objective To explore a sTable. method for common carotid artery intubation in New Zealand rabbits that is secure and enables continuous and regular blood collection. Methods The left common carotid artery of anaesthetized rabbits was dissected after successful puncture using a BD closed venous indwelling needle (type Y), followed by ligation and fixing with silk suture. Then, the sternocleidomastoid muscle and sternohyoid muscle were sutured in the front, middle, and back of the needle to stop the intubation from exiting. The catheter was routed close to the skin around the neck and sutured at the skin at the back of the neck. Results The rate of blocking of the carotid artery catheter in the control group was 35%, while that in the other group was 5%. In the control group, the rate of falling off the carotid artery catheter was 30%, while it did not fall off in the experimental group. There were significant differences in the incidence rate between the two groups in terms of common carotid artery catheter embolism and falling off. Conclusions Suturing and fixing a BD needle of the left common carotid artery in New Zealand rabbits at three different points was effective for stably restricting movement of the BD needle in all directions. This approach is durable and enables continuous blood collection, providing a foundation for successful experiments.

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History
  • Received:January 14,2018
  • Revised:
  • Adopted:
  • Online: September 17,2018
  • Published: