Parkinson’s disease (PD) is a neurodegenerative disease common in middle-aged and old people. It isdifficult to diagnose and differentiate PD, Parkinson’ s superimposition syndrome and other motor disorders in the earlystages. Therefore, preclinical research is particularly important, and animal molecular imaging are used. Imaging technologycan dynamically observe the formation of Parkinson’ s disease and the changes of dopamine receptor metabolism andkinetics before and after deep brain stimulation ( DBS) treatment in vivo. It is helpful for the early diagnosis andunderstanding pathogenesis of Parkinson’s disease. At present, small animal PET/ CT,MRI and SPECT are used for thebrain imaging of Parkinson’ s experimental animals for accurate diagnosis. Further research is needed to significantlyimprove the diagnostic efficiency and achieve a high degree of consistency with clinical diagnostic results, and serves thediagnosis and differential diagnosis of Parkinson’s disease and Parkinson’s syndrome. This paper reviews the results ofpreclinical studies on Parkinson’ s disease using molecular imaging technology in recent years, and illustrates thedevelopment trend of imaging medicine, which has a certain reference value for the future diagnosis and treatment of Parkinson’s disease.