Objective To examine the expression level and distribution of TRIM26 in multiple human and mouse tissues and organs. Methods The immunohistochemistry ( IHC) PV- 9000 two-step method ( non-biotin) was used to determine the expression and distribution of TRIM26. BioGPS was used to calculate TRIM26 expression levels in human tissues and organs, whereas RT-qPCR was performed to determine its counterparts in mice. Results The IHC result indicated that the TRIM26 protein is expressed in the cytoplasm and nucleus, mainly in epithelial cells. Its expression distribution in human esophageal squamous cells, gastric epithelial cells, small intestinal epithelial cells, colon epithelial cells, islet cells, brain glial cells, renal tubular epithelial cells, glomerular cells, and endometrial glandular epithelial cells was consistent with that in the mouse equivalent cells. The highest relative expression of TRIM26 was observed in human tracheal cells and mouse spleen. Conclusions Humans and mice show consistent expression distribution of TRIM26 in multiple tissues and organs, but with different expression levels. Revealing the TRIM26 expression profile in human and mouse main organs and tissues lays a foundation for further exploration of the functions of TRIM26 in different tissues and organs by engineering conditional TRIM26-knockout mice.