In vitro screening of mouse bone marrow-derived dendritic cells induced by five plant polysaccharides
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1.College of Life Sciences and Bioengineering, Beijing University of Technology, Beijing 100124, China. 2. College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124. 3. Institute of Chinese Materia Medica;China Academy of Chinese Medical Sciences, Beijing 100700

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R-33

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    Abstract:

    Objective To investigate the adjuvant activity of polysaccharides from Ganoderma lucidum, Lycium barbaru, Versicolor, Astragalus and Lentinan on bone marrow-derived dendritic cells ( BMDCs) from C57BL/ 6 mice. Methods Bone marrow cells from C57BL/ 6 mice were cultured in the presence of granulocyte-macrophage colony- stimulating factor GM-CSF. After 6 days, immature BMDCs were collected and co-cultured with the polysaccharides of G. lucidum, L. barbaru, Versicolor, Astragalus or Lentinula. CpG oligodeoxynucleotides were used as a positive control. The cell surface expression of CD80, CD86 and MHCII were detected by fluorescence-activated cell sorting analysis. The release of cytokines interleukin (IL)-6, IL-12 and tumor necrosis factor TFN-α were measured by enzyme-linked immunosorbent assay. Results Versicolor polysaccharides significantly upregulated the surface expression of CD80, CD86 and MHCII on BMDCs, as well as the release of the cytokines IL-6, IL-12 and TFN-α compared with the control group. Conclusions Versicolor polysaccharides promoted the maturation of BMDCs in vitro and may have potential as an adjuvant for vaccine preparations.

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History
  • Received:February 21,2020
  • Revised:
  • Adopted:
  • Online: December 25,2020
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