Objective To investigate the effect of resveratrol on the repair of hypoxic brain injury through the HIF-1α/ BNIP3-mediated autophagy signaling pathway in mice. Methods A mouse model of intermittent hypoxia was established using the CQY-1 small animal hypoxia detection system. The hypoxia tolerance time, respiratory rate, and heart rate changes of the mice were detected. Histopathological staining was used to detect morphological changes to the mice brain. Western blot was used to detect the protein expression levels of HIF-1α, P53, BNIP3, Beclin 1, LC3 and P62 in mice brain tissue. Results Compared with the findings in the hypoxia group, resveratrol prolonged the hypoxia tolerance time; increased the respiratory rate and reduced the heart rate of hypoxic mice; alleviate brain tissue damage in the hippocampus; down-regulated HIF-1α, P53, Beclin 1 and LC3 protein expression; and up-regulated P62 protein expression. Conclusions Resveratrol down-regulates HIF-1α/ BNIP3-mediated autophagy signaling pathway gene expression to promote the repair of hypoxic brain injury in mice.