【Abstract】 Objection To determine the optimal anesthetic method, our study compared the effect of three anesthetic methods in orthopedic animal experiments. Methods 80 experimental animals were randomly divided into three groups, group A was given with sumianxin II by intramuscular injection, group B was administrated with 0.6% pentobarbital sodium by intravenous injection, and group C was given with 0.6% pentobarbital sodium by introvenous injection combined with sumianxin II by intramuscular injection. Anesthesia was performed in orthopedic animal experiments surgery. Induction period of anesthesia (maintenance of anesthesia time), the recovery period, the anesthetic effect, mortality rates were observed after anesthesia. Results Anesthesia induction period is long, the effect is not good in group A; anesthesia induction period was short, but the bad effects of anesthesia and the mortality rate was high in group B; however, induction period of group C was short and safety. Conclusion pentobarbital sodium combined with sumianxin II is a good anesthetic method compared with pentobarbital sodium or sumianxin II respectively. Its induction period is short and safety. Therefore, it is an excellent and safe method to anesthetize animal in orthopaedic using Sumianxin II combined with pentobarbital sodium. 【Keywords】 Sumianxin II; pentobarbital sodium;experimental animal;anesthesia