Tree shrews is similar to human beings in biological characteristics, physiology and biochemistry, metabolism, genome and so on, it has played an important role in many scientific research fields, such as physiology, pathology, virology, immunology, pharmacology, genetics and so forth . To this day most of wild tree shrews were used in biomedical field, there is no national quality control standard. In order to assure standardization and scientific of tree shrews introduction, domestication, alimentation, reproduction, quality control, animal welfare, and increase reproduction rate and the survival rate of tree shrew. According to requirement of veterinary public health and laboratory animal sciences, this paper summarized some basic principles, technical operation and welfare requirements relate to tree shrews, which includes wild catching ,transportation, quarantine, feeding , breeding and others, it is beneficial to interrelated workers and correlation studies.